Effect of density on performance and some hemolymph antioxidant parameters of white-leg shrimp (Litopenaeus vannamei) in a biofloc system

Document Type : Research Paper

Authors

1 Ph.D. Student in Fisheries, Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Associate Professor in Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Associate Professor in Department of Fisheries, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran

4 Assistant Professor in Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

The present study was designed to investigate the effect of density on some performance and activity of biochemical parameters of white-leg shrimp (Litopenaeus vannamei). Twenty-day-old larvae (PL30) were distributed at densities of 100, 200, 300 and 400 No. m-2 in twelve polyethylene tanks (working volume of 200 L) containing bioflocks and fed ad libitum using artificial feed. Each triplicate tank was assigned to one treatment. At the end of experiment, performance indicators, antioxidant enzymes activities and some of the stress parameters were determined. The results showed that an increase in the shrimp density under the biofloc system had a significant effect on the growth performance and hemolymph biochemical properties of white-leg shrimp and declined the final weight, daily growth rate and feeding efficiency ratio. However, the amount of biomass increased with increasing density and reached to more than 1.7 folds higher than that in under the density of 100 No. m-2. However, the antioxidant defense parameters including the activity level of superoxide dismutase, catalase and glutathione peroxidase enhanced with increasing the shrimp density in the biofloc system. Cortisol and glucose levels of the hemolymph also showed a decreasing trend with increasing density to 300 No. m-2,

Keywords


Andrade T., Afonso A., Perez-Jimenez A., Oliva-Teles A., De Las Heras V., Mancera J.M., Serradeiro R. and Costas B. 2015. Evaluation of different stocking densities in a Senegalese sole (Solea senegalensis) farm: Implications for growth, humoral immune parameters and oxidative status. Aquaculture, 438: 6–11.
Araneda M., Perez E.P. and Gasca-Leyva E. 2008. White shrimp Penaeus vannamei culture in freshwater at three densities: Condition state based on length and weight. Aquaculture, 283(1-4): 13–18.
Arcos G.F., Ibarra A.M., Vazquez‐Boucard C., Palacios E. and Racotta I.S. 2003. Haemolymph metabolic variables in relation to eyestalk ablation and gonad development of Pacific white shrimp Litopenaeus vannamei Boone. Aquaculture Research, 34(9): 749–755.
Bardera G., Owen M.A., Facanha F.N., Alcaraz-Calero J.M., Alexander M.E. and Sloman K.A. 2021. The influence of density and dominance on Pacific white shrimp (Litopenaeus vannamei) feeding behaviour. Aquaculture, 531: 1–12 (735949).
Bardera G., Usman N., Owen M., Pountney D., Sloman K.A. and Alexander M.E. 2019. The importance of behaviour in improving the production of shrimp in aquaculture. Reviews in Aquaculture, 11(4): 1104–1132.
Bateman K.S. 2021. Viruses affecting crustaceans. P: 305–340. In: Hurst C.J. (Ed.). Studies in Viral Ecology. John Wiley and Sons, USA.
Battisti E.K., Rabaioli A., Uczay J., Sutili F.J. and Lazzari R. 2020. Effect of stocking density on growth, hematological and biochemical parameters and antioxidant status of silver catfish (Rhamdia quelen) cultured in a biofloc system. Aquaculture, 524: 1–13 (735213).
Biagini G., Sala D. and Zini I. 1995. Diethyldithiocarbamate, a superoxide dismutase inhibitor, counteracts the maturation of ischemic-like lesions caused by endothelin-1 intrastriatal injection. Neuroscience Letters, 190(3): 212–216.
Briggs M., Funge-Smith S., Subasinghe R. and Phillips M. 2004. Introductions and Movement of Penaeus vannamei and Penaeus stylirostris in Asia and the Pacific. RAP Publication, Thailand. 92P.
Cardona E., Saulnier D., Lorgeoux B., Chim L. and Gueguen Y. 2015. Rearing effect of biofloc on antioxidant and antimicrobial transcriptional response in Litopenaeus stylirostris shrimp facing an experimental sub-lethal hydrogen peroxide stress. Fish and Shellfish Immunology, 45(2): 933–939.
Castex M., Lemaire P., Wabete N., and Chim L. 2009. Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris. Aquaculture, 294(3-4): 306–313.
Chen M., Chen X.Q., Tian L.X., Liu Y.J. and Niu J. 2020. Beneficial impacts on growth, intestinal health, immune responses and ammonia resistance of pacific white shrimp (Litopenaeus vannamei) fed dietary synbiotic (mannan oligosaccharide and Bacillus licheniformis). Aquaculture Reports, 17: 1–11 (100408).
Da Silveira L.G.P., Rosas V.T., Krummenauer D., Poersch L.H., and Wasielesky Jr. W. 2022. Comparison between horizontal and vertical substrate in shrimp super-intensive culture in bioflocs system. Aquacultural Engineering, 96: 1–7 (102218).
De Costa F.P., De Farias Gomes B.S.F., Pereira S.D.N.A. and De Fatima Arruda M.D.F. 2016. Influence of stocking density on the behaviour of juvenile Litopenaeus vannamei (Boone, 1931). Aquaculture Research, 47: 912–924.
Dewi N.R., Huang H.T., Wu Y.S., Liao Z.H., Lin Y.J., Lee P.T. and Nan F.H. 2021. Guava (Psidium guajava) leaf extract enhances immunity, growth, and resistance against Vibrio parahaemolyticus in white shrimp Penaeus vannamei. Fish and Shellfish Immunology, 118: 1–10.
Dong J., Zhao Y.Y., Yu Y.H., Sun N., Li Y.D., Wei H., Yang Z.Q., Li X.D. and Li L. 2018. Effect of stocking density on growth performance, digestive enzyme activities, and nonspecific immune parameters of Palaemonetes sinensis. Fish and Shellfish Immunology, 73: 37–41.
El‐Sayed A.F.M. 2021. Use of biofloc technology in shrimp aquaculture: A comprehensive review, with emphasis on the last decade. Reviews in Aquaculture, 13(1): 676–705.
Flohe L. and Gunzler W.A. 1984. Assays of glutathione peroxidase. Methods in Enzymology, 105: 114–120.
Goth L. 1991. A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196(2-3): 143–151.
Ju Z.Y., Forster I., Conquest L. and Dominy W. 2008. Enhanced growth effects on shrimp (Litopenaeus vannamei) from inclusion of whole shrimp floc or floc fractions to a formulated diet. Aquaculture Nutrition, 14(6): 533–543.
Khanjani M.H. and Sharifinia M. 2020. Biofloc technology as a promising tool to improve aquaculture production. Reviews in Aquaculture, 12(3): 1836–1850.
Kim M.S., Min E., Kim J.H., Koo J.K. and Kang J.C. 2015. Growth performance and immunological and antioxidant status of Chinese shrimp, Fennerpenaeus chinensis reared in bio-floc culture system using probiotics. Fish and Shellfish Immunology, 47(1): 141–146.
Kim S.K., Pang Z., Seo H.C., Cho Y.R., Samocha T. and Jang I.K. 2014. Effect of bioflocs on growth and immune activity of Pacific white shrimp, Litopenaeus vannamei postlarvae. Aquaculture Research, 45(2): 362–371.
Klongklaew N., Praiboon J., Tamtin M. and Srisapoome P. 2021. Chemical composition of a hot water crude extract (HWCE) from Ulva intestinalis and its potential effects on growth performance, immune responses, and resistance to white spot syndrome virus and yellowhead virus in Pacific white shrimp (Litopenaeus vannamei). Fish and Shellfish Immunology, 112: 8–22.
Krummenauer D., Peixoto S., Cavalli R.O., Poersch L.H. and Wasielesky Jr. W. 2011. Superintensive culture of white shrimp, Litopenaeus vannamei, in a biofloc technology system in southern Brazil at different stocking densities. Journal of the World Aquaculture Society, 42(5): 726–733.
Li X., Dong S., Lei Y. and Li Y. 2007. The effect of stocking density of Chinese mitten crab Eriocheir sinensis on rice and crab seed yields in rice–crab culture systems. Aquaculture, 273(4): 487–493.
Li X., Han T., Zheng S. and Wu G. 2022. Hepatic glucose metabolism and its disorders in fish. P: 207–236. In: Wu G. (Ed.). Recent Advances in Animal Nutrition and Metabolism. Springer Nature, Switzerland.
Liu G., Zhu S., Liu D., Guo X. and Ye Z. 2017. Effects of stocking density of the white shrimp Litopenaeus vannamei (Boone) on immunities, antioxidant status, and resistance against Vibrio harveyi in a biofloc system. Fish and Shellfish Immunology, 67: 19–26.
Lorenzon S., Giulianini P.G., Libralato S., Martinis M. and Ferrero E.A. 2008. Stress effect of two different transport systems on the physiological profiles of the crab Cancer pagurus. Aquaculture, 278(1-4): 156–163.
Menaga M. and Felix S. 2020. Comparison of water exchange rate, feed economics and nutritional composition of aerobic microbial floc in indoor and outdoor culture of Penaeus vannamei. P: 49–62. In: Felix S., Samocha T. and Menaga M. (Eds.). Vannamei Shrimp Farming. CRC Press, UK.
Mishraa J.K., Samochaa T.M., Patnaika S., Speed M., Gandya R.L. and Alid A.M. 2020. Intensive nursery system for the Pacific white shrimp, Penaeus vannamei, under limited discharge condition. P: 159–180. In: Felix S., Samocha T. and Menaga M. (Eds.). Vannamei Shrimp Farming. CRC Press, UK.
Panigrahi A., Esakkiraj P., Das R.R., Saranya C., Vinay T.N., Otta S.K. and Shekhar M.S. 2021. Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus. Scientific Reports, 11(1): 1–13.
Panigrahi A., Sundaram M., Chakrapani S., Rajasekar S., Syama Dayal J. and Chavali G. 2019. Effect of carbon and nitrogen ratio (C:N) manipulation on the production performance and immunity of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in a biofloc‐based rearing system. Aquaculture Research, 50(1): 29–41.
Perazzolo L.M., Gargioni R., Ogliari P. and Barracco M.A. 2002. Evaluation of some hemato-immunological parameters in the shrimp Farfantepenaeus paulensis submitted to environmental and physiological stress. Aquaculture, 214(1-4): 19–33.
Poersch L., Brunson J., Gaona C.A., Stokes A., Richardson J., Pitts K. and Leffler J. 2021a. Pacific white shrimp, red drum, and tilapia integrated in a biofloc system: Use of tilapia as a consumer of total suspended solids. Journal of the World Aquaculture Society, 52(6): 1168–1177.
Poersch L.H., Magalhaes V., Lara G., Chaves F., Wasielesky W. and Foes G.K. 2021b. Comparative strategies for intensive shrimp production in ponds using biofloc technology system in Southern Brazil: Water quality, zootechnical performance and economic viability for Litopenaeus vannamei. Aquaculture Research, 52: 3713–3722.
Poli M.A., Martins M.A., Pereira S.A., Jesus G.F.A., Martins M.L., Mourino J.L.P. and Do Nascimento Vieira F. 2021. Increasing stocking densities affect hemato-immunological parameters of Nile tilapia reared in an integrated system with Pacific white shrimp using biofloc technology. Aquaculture, 536: 1–14 (736497).
Qiao G., Zhang M., Li Y., Xu C., Xu D.H. and Zhao Z. 2018. Biofloc technology (BFT): An alternative aquaculture system for prevention of Cyprinid herpesvirus infection in gibel carp (Carassius auratus gibelio). Fish and Shellfish Immunology, 83: 140–147.
Romano N. and Zeng C. 2017. Cannibalism of decapod crustaceans and implications for their aquaculture: A review of its prevalence, influencing factors, and mitigating methods. Reviews in Fisheries Science and Aquaculture, 25(1): 42–69.
Shourbela R.M., Khatab S.A., Hassan M.M., Van Doan H. and Dawood M.A. 2021. The effect of stocking density and carbon sources on the oxidative status, and nonspecific immunity of Nile tilapia (Oreochromis niloticus) reared under biofloc conditions. Animals, 11(1): 1–13 (184).
Suantika G., Situmorang M.L., Saputra F.I., Putri S.L.E., Putri S.P., Aditiawati P. and Fukusaki E. 2020. Metabolite profiling of whiteleg shrimp Litopenaeus vannamei from super-intensive culture in closed aquaculture systems: A recirculating aquaculture system and a hybrid zero water discharge-recirculating aquaculture system. Metabolomics, 16(4): 1–11.
Tacon A.G., Jory D. and Nunes A. 2013. Shrimp feed management: Issues and perspectives. On-farm feeding and feed management in Aquaculture, 583: 481–488.
Taukhid I., Tampangallo B.R., Tahe S. and Undu M.C. 2021. The application of progressive systems in high density vannamei shrimp culture. In IOP Conference Series: Earth and Environmental Science, 860(1): 1–16 (012022).
Tintos A., Miguez J.M., Mancera J.M. and Soengas J.L. 2006. Development of a microtitre plate indirect ELISA for measuring cortisol in teleosts, and evaluation of stress responses in rainbow trout and gilthead sea bream. Journal of Fish Biology, 68(1): 251–263.
Wang Z., Zhang Y., Yao D., Zhao Y., Tran N.T., Li S., Ma H. and Aweya J.J. 2021. Metabolic reprogramming in crustaceans: A vital immune and environmental response strategy. Reviews in Aquaculture, 14(3): 1094–1119.
Xu W., Xu Y., Su H., Hu X., Xu Y., Li Z., Wen G. and Cao Y. 2020. Effects of feeding frequency on growth, feed utilization, digestive enzyme activity and body composition of Litopenaeus vannamei in biofloc-based zero-exchange intensive systems. Aquaculture, 522: 1–14 (735079).
Xu W.J. and Pan L.Q. 2013. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture, 412: 117–124.
Xue S., Ding J., Li J., Jiang Z., Fang J., Zhao F. and Mao Y. 2021. Effects of live, artificial and mixed feeds on the growth and energy budget of Penaeus vannamei. Aquaculture Reports, 19: 1–6 (100634).
Yong A.S.K., Mok W.Y., Tamrin M. L. M., Shapawi R. and Kim Y.S. 2020. Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition. Aquaculture Research, 51(6): 2252–2260.