Interspecific gynogenesis induction in Siberian sturgeon (Acipenser baerii ) using heterologous sperm

Document Type : Research Paper

Authors

1 Scientific Member in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran

2 Assistant Professor in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran

3 Professor in Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

4 - Associate Professor in Genetics and Biotechnology Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran

5 M.Sc. in Fisheries, Aquaculture Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran

6 M.Sc. in Fisheries, Health and Disease Department, International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran

10.22124/japb.2021.20693.1439

Abstract

Siberian sturgeon (Acipenser baerii) grows rapidly in breeding ponds. Gynogenesis is a suitable technique for producing sturgeon female sex. The aim of this study was to establish female sex in Siberian sturgeon using induction of gynogenesis. The sperm of Persian sturgeon (Acipenser persicus) was genetically inactivated by UV radiation at a dose of 8514 J/m2. In this case, sperm with 40% motility were combined with Siberian sturgeon oocytes and in 2°C cold shock for 30 minutes, 10 minutes after activity, gynogenic progeny were created. Fertilization rate was 44.5% (control hybrid 62%) and hatching rate was 19.8% (control hybrid 40.5%). Purely maternal heritability in gynogeneic progeny was confirmed using microsatellite markers. By achieving the biotechnique of sperm production with inactivated DNA of Persian sturgeon, it is possible to prevent the risk of extinction and protect other sturgeon by inducing gynogenesis.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 10, Issue 3
January 2023
Pages 23-48

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