Testing beluga molecular sex determination by amplification of female specific genomic region

Document Type : Research Paper

Authors

1 Assistant Professor in Department of Genetics and Biotechnology, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

2 Assistant Professor in Department of Physiology and Biochemistry, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

3 Ph.D. in Fisheries Science, Department of Physiology and Biochemistry, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

4 Assistant Professor in Department of Fish Health and Diseases, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

5 Scientific Member in Department of Genetics and Biotechnology, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

10.22124/japb.2022.21408.1454

Abstract

In sturgeon aquaculture, sex determination is a major challenge since culture of female sturgeon, as a valuable source of caviar production, is economically important and the differentiation of male and female sturgeons is indeed necessary to reduce costs. Indirect evidence shows that the sex determination system in sturgeon is ZW and the sex of the female determines the sex. The situation which underlies locus discovery involved in sturgeon sex determination in genomic studies. The current study, for the first time in Iran, aimed to determine the beluga sex using the above-mentioned locus specific primers. For this purpose, caudal fins samples were collected from 23 beluga broodstock (14 females and 9 males). Genomic DNA was extracted by the ammonium acetate method. After quantitative and qualitative evaluation of the extracted DNA, specific primers were used to amplify a small fragment of the female beluga locus. Based on the obtained results, the single fragment slightly larger than 100 bp in size was amplified in 14 females while no specific amplification was observed in the male. Therefore, the molecular test can be used as a reliable, efficient, relatively low-cost, and non-invasive method for early sex determination in beluga in Iran.

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References

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Aquatic Physiology and Biotechnology
Volume 10, Issue 4
March 2022
Pages 81-94

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