Investigation on susceptibility of the Caspian kutum (Rutilus kutum ) primary caudal fin cells to spring viremia of carp virus

Document Type : Research Paper

Authors

1 M.Sc. Student in Fisheries, Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran

2 Assistant Professor in Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran

3 Assistant Professor in Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran

10.22124/japb.2022.21587.1456

Abstract

In this in vitro study the susceptibility of primary caudal fin cells of Caspian Whitefish (Rutilus kutum)- as the mast commercial endemic fish in the southern Caspian Sea- was examined to the spring viremia of carp virus (SVCV), the acute infectious, hemorrhagic and contagious agent. For this purpose, the primary cell culture was established from the caudal fin of fingerlings, using tissue explant technique and trypsinization of minced tissues. After the propagation of SVCV’s standard strain, the different dilutions of virus, in six replicates, were inoculated to 96-well plates which was contained primary caudal fin monolayer of cells after 5 passages, and the EPC cell line. In order to evaluation of virus characterization, an indirect immunofluorescent antibody test was hired. The clearly observable cytopathic effects and emergence of SVCV antigens as light fluorescence spots in both primary culture of caudal fin cells and the EPC cell line, highly confirmed the susceptibility of caudal fin primary cells to the SVCV.

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References

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Aquatic Physiology and Biotechnology
Volume 11, Issue 1
June 2023
Pages 1-18

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