CYP1A gene expression and antioxidant enzymes in green scat (Scatophagus argus) as PAHs biomarker

Document Type : Research Paper


1 Assistant Professor in Department of Biotechnology, Persian Gulf Institute, Persian Gulf University, Bushehr, Iran

2 M.Sc. Student in Fisheries, Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran



Monitoring of the pollutant effects in the biological environment requires the identification of a suitable biomarker. In this study, the efficacy of the expression of the CYP1 gene and the antioxidant enzymes in the Scatophagus argus were studied as biomarker of oil pollutants at three stations (Bandargah, Solhabad and Jofreh) and the control samples. CYP1A gene expression in liver was significantly different from control samples in all regions. The highest CYP1 gene expression was measured in Solhabad station. CYP1A gene expression in the gills of green scat fish showed a significant increase compared to control samples. The highest and lowest CYP1A gene expression was observed in the gills in Solhabad and Bandargah samples, respectively. The amount of superoxide dismutase and catalase of liver tissue in Jofrah and Solhabad stations were significantly lower than the control (P<0.05). In contrast, the rate of lipid peroxidation and GST were directly correlated with the amount of polycyclic aromatic hydrocarbons (P<0.05, R=0.96). Due to the relationship between CYP1A gene expression and antioxidant markers and the level of contamination, CYP1A gene expression and antioxidant markers can be used as a suitable biomarker for PAH contaminants in this species.


Main Subjects

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