Document Type : Research Paper
University of Hormozgan
Persian Gulf and Oman Sea Research Center
One of the most important secondary metabolites of the marine sponges are steroids. The aim of this study was to investigate the antifungal activity of the steroidal fractions derived from the Persian Gulf sponge Axinella sinoxea. Solvents with different polarities did extraction and then the fractions were separated through column chromatography with silica gel. Identification of steroidal fractions were done by thin layer chromatography and gas chromatography coupled with mass spectrometry. Then, antifungal properties of the steroids were investigated through determining the minimum inhibition concentration and minimum fungicidal concentration by tubular dilution method against Aspergillus fumigatus and Candida albicans. The identified steroids included compounds of Stigmasta, Ergosta, Cholest and Norgorgosta, which showed different results regarding the growth inhibition and fungicidal effects against the microorganisms at different experimental doses. Stigmasta and Ergosta showed more antifungal effects against A. fumigatus compared with the other steroids and Ergosta caused the best inhibitory and killing effects against C. albicans compared with the other steroids. In conclusion, promising results were found regarding the antimicrobial effects of the extracted steroids of the marine sponge A. sinoxea from Larak island, revealing the necessity of more comprehensive investigations for the synthesis of biomedicines from the marine bioactive compounds.