The response of Anabaena sp. to gibberellic acid

Document Type : Research Paper


1 Associate Professor in Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran

2 M.Sc. in Plant Physiology, Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran



In this study, the effect of gibberellic acid on vegetative and heterocyst cell size, inducing sugar and protein content was investigated in Anabaena at three durations (7, 14 and 21 days). According to the results, vegetative cell size did not show any significant differentiation under gibberellic acid treatments and time duration. Length and width of heterocyst cells showed a significantly different impact only with 10µM gibberellic acid at 7-day-old cultures. Algae treated with gibberellic acid had more fresh weight. Gibberellic acid induced heterocyst formation after 14 and 21 days. The amount of photosynthetic pigments increased in the 7 and 14-day cultures and decreased in the 21-day cultures. Application of gibberellic acid to the culture medium did not affect heterocyst percentage after 7 days, but it increased the heterocyst numbers in the 14 and 21-day cultures. Reducing sugar content changed dependence of gibberellic acid concentration and growth period. Treatment with gibberellic acid could increase protein content at different concentration and growth period. The results of this research showed notable effect of this plant growth regulator on Anabaena.


Main Subjects

Adair O.V. and Miller M.W. 1982. Growth responses of the diatome, Cyclotella cryptica (Bacilariophyceae), to gibberellic acid. Journal of Phycology, 18: 587–589.
Arora S. and Mishra G. 2019. Biochemical modulation of Monodopsis subterranea (Eustigmatophyceae) by auxin and cytokinin enhances eicosa-pentaenoic acid productivity. Journal of Applied Phycology, 31(6): 3441–3452.
Bradford M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Journal of Analytical Biochemistry, 72: 248–254.
Falkowska M., Pietryczuk A., Piotrowska A., Bajguz A., Grygoruk A. and Czerpak R. 2011. The effect of gibberellic acid (GA3) on growth, metal biosorption and metabolism of the green algae Chlorella vulgaris (Chlorophyceae) Beijerinck exposed to cadmium and lead stress. Polish Journal of Environmental Studies, 20: 53–59.
Golden J.W. and Yoon H.S. 2003. Heterocyst development in Anabaena. Current Opinion in Microbiology, 6(6): 557–563.
Han X., Zeng H., Bartocci P., Fantozzi F. and Yan Y. 2018. Phytohormones and effects on growth and metabolites of microalgae: A review. Fermentation, 4: 1–15.
Hosseini Madani N.S., Shamsaie Mehrgan M., Hosseini Shekarabi S.P. and Pourang N. 2021. Regulatory effect of gibberellic acid (GA3) on the biomass productivity and some metabolites of a marine microalga, Isochrysis galbana. Journal of Applied Phycology, 33: 255–262.
Kannaiyan S., Aruna S.J., Kumari S.M.P. and Hall D.O. 1997. Immobilized cyanobacteria as a biofertilizer for rice crops. Journal of Applied Phycology, 9: 167–174.
Kovalev N.N., Leskova S.Y., Mikheev Y.V., Pozdnyakova Y.M. and Esipenko R.V. 2022. The effect of gibberellic acid on the production characteristics and biochemical parameters of Tetraselmis suecica in an enrichment culture. KnE Life Sciences, 228–235.
Lin B., Ahmed F., Du H., Li Z., Yan Y., Huang Y., Cui M., Yin Y., Li B. and Wang M. 2018. Plant growth regulators promote lipid and carotenoid accumulation in Chlorella vulgaris. Journal of Applied Phycology, 30: 1549–1561.
Madani N.S.H., Shamsaie Mehrgan M., Hosseini Shekarabi S.P. and Pourang N. 2021. Regulatory effect of gibberellic acid (GA3) on the biomass productivity and some metabolites of a marine microalga, Isochrysis galbana. Journal of Applied Phycology, 33(1): 255–262.
Mansouri H. and Talebizadeh B. 2016. Effect of gibberellic acid on the cyanobacterium Nostoc linckia. Journal of Applied Phycology, 28: 2187–2193.
Olszewsk N., Sun T. and Gubler F. 2002. Gibberellin signaling: Biosynthesis, catabolism, and response pathways. Plant Cell, 14: S61–80.
Pan X., Chang F., Kang L., Liu Y., Li G. and Li D. 2008. Effects of gibberellin A3 on growth and microcystin production in Microcystis aeruginosa (Cyanophyta). Journal of Plant Physiology, 165: 1691–1697.
Park W.K., Yoo G., Moon M., Kim C.W., Choi Y.E. and Yang J.W. 2013. Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtii cultivated for biodiesel production. Applied Biochemistry and Biotechnology, 171: 1128–1142.
Paster Z. and Abbott B.C. 1970. Gibberellic acid: A growth factor in the unicellular alga Gymnodinium breve. Science, 169(3945): 600–601.
Razem F.A., Baron K. and Hill R.D. 2006. Turning on gibberellin and abscisic acid signaling. Current Opinion in Plant Biology, 9: 454–459.
Somogyi M. 1952. Notes on sugar determination. Journal of Biological Chemistry, 195: 19–23.
Stewart I., Webb P.M., Schluter P.J. and Shaw G.R. 2006. Recreational and occupational field exposure to freshwater cyanobacteria- A review of anecdotal and case reports, epidemiological studies and the challenges for epidemiologic assessment. Environmental Health, 5(1): 1–13.
Stirk W.A., Balint P., Tarkowska D., Novak O., Maroti G., Ljung K., Tureckova V., Strnad M., Ordog V. and Van Staden J. 2014. Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Journal of Plant Biochemistry and Physiology, 79: 66–76.
Sukran D., Gunes T. and Sivaci R. 1998. Spectrophotometric determination of chlorophyll-a, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22(1): 13–18.
Tandeau De Marsac N. and Houmard J. 1993. Adaptation of cyanobacteria to environmental stimuli: New steps towards molecular mechanisms. FEMS Microbiology Reviews, 104: 119–190.
Vaishampayan A., Sinha R.P. and Hader D.P. 1998. Use of genetically improved nitrogen-fixing cyanobacteria in rice paddy fields: Prospects as a source material for engineering herbicide sensitivity and resistance in plants. Botanica Acta, 111: 176–190.
Whitton B.A. 2000. Soils and rice-fields. P: 233–255. In: Whitton B.A. and Potts M. (Eds.). The Ecology of Cyanobacteria. Kluwer Academic Publishers, Netherlands.
Whitton B.A. and Potts M. 2000. Introduction to the cyanobacteria. P: 1–11. In: Whitton B.A. and Potts M. (Eds.) The Ecology of Cyanobacteria: Their Diversity in Time and Space. Kluwer Academic, Netherlands.
Yu X.J., Sun J., Sun Y.Q., Zheng J.Y. and Wang Z. 2016. Metabolomics analysis of phytohormone gibberellin improving lipid and DHA accumulation in Aurantiochytrium sp. Biochemical Engineering Journal, 112: 258–268.