Evaluation of the effect of henna plant extract (Lawsonia inermis) compared to copper sulfate (CuSo4.5H2o) on blood-immunity, stress, antioxidant activity in gill and liver tissue of Siberian sturgeon (Acipenser baerii)

Barzgar, Ruhollah; Farokhrouz Lashidani, Masoud; Khara, Hossein; Ahmadnezhad, Mohaddeseh; Shenavar Masouleh, Alireza

doi:10.22124/japb.2023.24825.1502

Evaluation of the effect of henna plant extract (Lawsonia inermis) compared to copper sulfate (CuSo4.5H2o) on blood-immunity, stress, antioxidant activity in gill and liver tissue of Siberian sturgeon (Acipenser baerii)

Document Type : Research Paper

Authors

¹ Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran, PO Box 1616

² Department of Fishery, lahijan Branch, Islamic Azad University, Lahijan, Iran

³ Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences Research Institute, Bandar Anzali, Iran

⁴ International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

10.22124/japb.2023.24825.1502

Abstract

Henna plants (Lawsonia inermis) and copper sulfate (CuSo4.5H2o) are used for healing and repairing skin wounds due to their antibiotic and antifungal properties.. For this purpose, 500 pieces of Siberian fish with an average weight of 15±1.2 grams were used. First, the acute toxicity (LC50) and then the maximum permissible concentration (MAC) were determined. To experiment, 5 treatments with 3 replications were designed. In treatments, 1 to 4, doses equal to their values (MAC) and amounts equal to 25%, 50%, and 75% of MAC were used for each of the extracts. In treatment 5, copper sulfate at the rate of 0.07 mg/liter was affected by compounds with determined concentrations for 4 days (96 hours) to compare the effect. The results showed that henna plant extract performed better than copper sulfate in indicators such as white blood cell count, stress reduction, and liver enzyme activity. Finally, the present study showed that henna plant extract performed better and can be used as a suitable substitute for copper sulfate for disinfection in Siberian fish farms. Also, the results showed that henna plant extract has negative effects on the blood and biochemical indices of Siberian fish.

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