Investigating the cytotoxic properties of Chrysaora hysoscella jellyfish venom against human colon cancer cells

Mohammadzadeasl, Asghar; Zolgharnain, Hossein; Matroodi, Soheila; Tahoori, Fatemeh

doi:10.22124/japb.2024.26334.1522

Investigating the cytotoxic properties of Chrysaora hysoscella jellyfish venom against human colon cancer cells

Document Type : Research Paper

Authors

¹ Department of Marine Biology, Faculty of Marine and Ocean Sciences, Khorramshahr University of Marine Sciences and Arts, Khorramshahr, Iran

² Department of Marine Biology, Faculty of Marine and Ocean Sciences, Khorramshahr University of Marine Sciences and Technologies, Khorramshahr, Iran

10.22124/japb.2024.26334.1522

Abstract

Jellyfish venom contains a variety of compounds including several potential therapeutic properties. This paper investigates the cytotoxic properties by purifying the proteins in the jellyfish venom of Chrysaora hysoscella species. Then, the fractions of C. hysoscella jellyfish venom were purified using HPLC method, and then their molecular weight was determined by SDS-PAGE method. The cytotoxic properties of the fractions were evaluated using the MTT method against HCT116 and Caco-2 cancer cell lines as well as RAW 264.7 normal cells. The obtained results were analyzed using SPSS v.23 software, ANOVA, and Tukey tests. Based on the obtained results, the raw venom of C. hysoscella had 10 fractions with a molecular weight of 15 to 170 kilodaltons, of which fraction 2 had a strong cytotoxic effect against the studied cancer cell lines. The value of IC50 against HCT116 and Caco-2 cells was 63.21 and 64.36 μg/ml, respectively, while against RAW 264.7 cells, it was equal to 122.88 μg/ml. The obtained results revealed the specific cytotoxic property of fraction 2 against colon cancer cells, while its cytotoxic effect on normal cells was significantly less. These results introduce the mentioned fraction as a potential anticancer agent for colon cancer.

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