Study of Abramis brama (Berg, 1905) infection with Ligula intestinalis (Linnaeus, 1758) in Siah Darvishan River and survey of them molecular diversity by evaluation of the ribosomal ITS2 region

Document Type : Research Paper

Authors

Assistant Professor in Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

10.22124/japb.2023.24804.1501

Abstract

Ligula intestinalis (Lineaus1758) is one of the most common parasites of freshwater fish. This study was conducted to survey the possibility of infection of freshwater breams (Abramis brama) caught from Siahdarvishan River and their morphological and molecular characteristics were investigated. 75 breams, after dissection, all parts of their abdominal cavity completely were examined for the presence of the L. intestinalis plerocercoids. Then, the morphometric characteristics of parasites were investigated. In the next step, the definitive identification of the genus and species of parasitic samples was confirmed by identification keys. For molecular analysis, the samples were stored at -20°C. Then, molecular studies were done on 10 plerocercoids obtained from 10 separate fish by analysis of ITS2 gene. From 75 A. brama samples, 18 fish (24%) were infected by L. intestinalis. In this study, the minimum, mean and maximum size of the isolated plerocercoids were 8, 26 and 74cm, respectively. Sequencing of the ITS2 gene of L. intestinalis isolates with GenBank samples showed that these isolates had genetic differences in different hosts and based on the comparison of the ITS2 gene locus, and the presence of host-dependent species in the genus Ligula is probable.

Keywords

References

Ahmadiara E. 2017. Is Ligula intestinalis really a probable threat to public health? Journal of Food Quality and Hazards Control, 4: 36–36.
Ahmadiara E., Hosseini S.H., Jalousian F., Ebrahimzadeh Mousavi H.A., Sakhaiifar S. and Gerami Sadeghiyan A. 2013. The study of the plerocercoid of Diphyllobothriidae (Cestoda, Pseudophyllidea) in two cyprinid hosts, Abramis brama and Alburnoides bipunctatus from north and northwest of Iran. Iranian Journal of Veterinary Medicine, 7(2): 103–109. doi: 10.22059/IJVM.2013.35060
Arme C. 1997. Ligulosis in two cyprinid hosts: Rutilus rutilus and Gobio gobio. Journal of Helminthology, 34: 191–196. doi: 10.1079/JOH2002101
Barson M. and Marshall B.E. 2013. The occurrence of the tapeworm, Ligula intestinalis (L.), in Barbus paludinosus from a small dam in Zimbabwe. African Journal of Aquatic Science, 28: 1–4. doi: 10.2989/16085910309503782
Bouzid W., Sovan Lek M., Mace O., Ben Hassine R., Etienne R., Legal L. and Loot G. 2008. Genetic diversity of Ligula intestinalis (Cestoda: Diphyllobothriidae) based on analysis of inter-simple sequence repeat markers. Journal of Zoological Systematics and Evolutionary Research, 46: 289–296. doi: 10.1111/j.1439-0469.2008. 00471.x
Chubb J.C., Pool D.W. and Weltkamp C.J. 1987. A key to species of cestodes (tapeworms) parasitic in British and Irish freshwater fishes. Journal of Fish Biology, 31(4): 517–543. doi: 10.1111/j.1095-8649.1987.tb05256.x
Dubinina M.N. 1980. Tapeworms (Cestoda, Ligulidae) of the Fauna of the USSR. Amerind Publishing Company, UK. 320P.
Eslami A. 2006. Veterinary Helminthology, Vol.: Cestoda (In Persian). University of Tehran Press, Iran. 328P.
Hajirostamloo M. 2008. The occurrence and parasite host of Ligula intestinalis in Sattarkhan Lake (East Azerbaijan-Iran). Journal of Animal and Veterinary Advances, 7: 221–225.
Jalali B. and Barzegar M. 2006. Fish parasite in Zarivar Lake. Journal of Agriculture Science and Technology, 8: 47–59.
Kennedy C.R. and Burrough R.J. 1981. The establishment and subsequent history of a population of Ligula intestinalis L. in roach Rutilus rutilus (L.). Journal of Fish Biology, 19: 105–126. doi: 10.1111/j.1095-8649.1981.tb05815.x
Koyun M. 2006. The seasonal effects of Ligula intestinalis L. (Cestodes: Pseudophyllidea) on Alburnus alburnus (Cyprinidae). International Journal of Zoological Research, 2: 75–76. doi: 10.3923/ijzr.2006.73.76
Li J. and Liao X. 2003. The taxonomic status of Digramma (Pseudophyllidae: Ligulidae) inferred from DNA sequences. Journal of Parasitology, 89: 792–799. doi: 10.1645/GE-3078
Liao X.H. and Liang Z.X. 1987. Distribution of ligulid tapeworms in China. Journal of Parasitology, 73: 36–48. doi: 10.2307/3282341
Logan F.J., Horak A., Stefka J., Aydogdu A. and Scholz T. 2004. The phylogeny of diphyllobothriid tapeworms (Cestoda: Pseudophyllidea) based on ITS-2 rDNA sequences. Parasitology Research, 94(1): 10–15. doi: 10.1007/s00436-004-1164-y. Epub 2004 Jul 27. PMID: 15278441
Loot G., Lek S., Dejean D. and Guegan J.F. 2002. Parasite-induced mortality in three host populations of the roach Rutilus rutilus (L.) by the tapeworm Ligula intestinalis (L.). Journal of Ecology of Freshwater Fish, 11: 168–177. doi: 10.1051/limn/2001010
Luo H.Y., Nie P., Yao W.J., Wang G.T. and Gao Q. 2003. Is the genus Digramma synonymous to the genus Ligula (Cestoda: Pseudophyllidea)? Parasitology Research, 89: 419–421. doi: 10.1007/s00436-002-0802-5
Pazooki J., Masoumian M., Yahyazadeh M. and Abbasi J. 2007. A Report on occurrence and parasite-host of Ligula intestinalis in Sattarkhan Lake (East Azerbaijan-Iran). Journal of Agricultural Science and Technology, 9: 25–33. doi: 10.5281/zenodo.1331091
Rahmati-Holasoo H., Hajimohammadi B., Ahmadiara E., Ebrahimzadeh Mousavi H.A., Rostami-Bashman M., Haghighi Khiabanian Asl A., Haghdoost I.S., Shokrpoor S. and Ghorbanalipour A.A. 2011. Study of infestation of Alburnoides bipunctatus with Ligula intestinalis in Latian Reservoir Dam Lake, Tehran Province, Iran: A histopathological study. Human and Veterinary Medicine International Journal of the Bioflux Society, 3: 18–24.
Rouis S.O., Rouis A.O., Dumont H.J., Magellan K. and Arab A. 2016. Dynamics and effects of Ligula intestinalis (L) infection in the native fish Barbus callensis Valenciennes, 1842 in Algeria. Acta Parasitologica, 61: 307–318. doi: 10.1515/ap-2016-0041
Sac G., Serezli E.E. and Okgerman H. 2016. The Occurrence of Ligula intestinalis in its fish host Rutilus rutilus (L) and the effects of parasite on the fish growth (Buyukckmece Reservoir, Turkey). Journal of Aquaculture Engineering and Fisheries Research, 2: 142–150. doi: 10.3153/JAEFR16016
Vossoughi G.H. and Mostajeer M.S. 2004. Freshwater Fish (In Persian). Tehran University Press, Iran. 334P.
Aquatic Physiology and Biotechnology
Volume 11, Issue 4
April 2024
Pages 1-14

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