Document Type : Research Paper
Authors
1
Department of Fisheries, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Shahrekord, Iran
2
1. Department of Fisheries and Environmental Sciences, Faculty of Natural Resourses and Earth Sciences, Shahrekord University, Shahrekord, Iran.
3
4. MSc in Fishery field, Department of Fisheries and Environmental Sciences, Faculty of Natural Resourses and Earth Sciences, Shahrekord University, Shahrekord, Iran.
10.22124/japb.2024.26606.1528
Abstract
The objective of this study was to investigate the possibility of inducing gynogenesis in the Bloody Parrot fish using irradiated sperm and thermal shock. For this purpose, 20 female Parrot fish and 3 male Flowerhorn fish (Grade A), which have the potential to produce reproductive hybrids, were used. Initially, the sperm genome obtained from the Flowerhorn fish was damaged using ultraviolet radiation at a wavelength of 254 nm and with a radiation dose of 30 J/m2, after which it was used to fertilize the eggs obtained from the Parrot fish. Thermal shock (9±0.5°C) was applied for 30 minutes to restore diploidy 4.5 minutes after fertilization. For assess gynogenesis, phenotypic analysis was conducted, examining the eye color and body shape of the offspring fish. Based on the obtained results, the hatching and larval survival rates of the offspring fish in the irradiated sperm group were significantly lower than those of the control group. Only 15.78% of the offspring in the irradiated sperm treatment showed complete resemblance to the maternal parent (p ≤ 0.05). According to the results, the immediate impact of ultraviolet radiation and thermal shock on the embryo and larval survival rates was more pronounced immediately after fertilization and decreased after hatching. In general, this study demonstrated the possibility of inducing gynogenesis in Parrot fish using ultraviolet radiation. However, increasing the hatching rate and the survival rate of the produced gynogenetic individuals requires further investigation. Therefore, examining different doses of ultraviolet radiation with varying exposure durations and utilizing different temperatures during thermal shock treatments is recommended to enhance the efficiency of gynogenesis induction in the future .
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