Evaluation of antioxidant and antibacterial effects of lotus plant extract (Nelumbo nucifera )

Alijani Ardeshir, Rashid; Kohanrouz Rostami, Zahra; Miri, Seyede Fatemeh; Gholami, Dariush

doi:10.22124/japb.2024.27651.1544

Evaluation of antioxidant and antibacterial effects of lotus plant extract (Nelumbo nucifera )

Document Type : Research Paper

Authors

¹ Department faculty

² Graduated student in Department of Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran

³ Assistant professor in Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.

10.22124/japb.2024.27651.1544

Abstract

Despite the high potential that aquatic plants can have in the field of biotechnology, few studies have been focused on this area. This study investigates the antibacterial and antioxidant effects of the ethanolic extract obtained from the seeds of the wetland plant lotus (Nelumbo nucifera). The antioxidant activity of this extract was evaluated using various tests including iron reduction potential, phenol content and DPPH radical scavenging activity. Antibacterial activity was evaluated using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. The results indicated high antioxidant effects (EC50:226.8 µg/mL) of the extract and increased iron reduction potential and phenol content (192.93 mg of gallic acid per g of extract). Antibacterial activity against Escherichia coli and Staphylococcus aureus was observed (at the concentrations of 50 µg/mL) in which the increase in the concentration of the extract led to the increase of its antibacterial effects significantly (P<0.05). However, despite the halo formation for the antibiotic disc, no halo was observed for the disc containing the concentrations of 200, 400 and 800 µg/ml extract. This study also analyzed the flavonoid content (50.3 mg of quercetin per g of extract) of the extract. These findings showed that the ethanolic extract of lotus seeds not only has antioxidant properties, but the use of appropriate concentrations can reduce the growth of bacteria, open a window to confirm future studies on the extract of this plant for use in traditional medicine and pharmaceutical industries

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