Document Type : Research Paper
Authors
1
Offshore Fisheries Research Center, National Institute of Fisheries Research Sciences, Agricultural Research, Education and Extension Organization, Chabahar, Iran
2
Iranian Fisheries Research Institute
3
Off-shore Fisheries Research Center- Chabahar, Iranian Fisheries Science Research, Institute (IFSRI), Agricultural Research Educations and Extension Organization (AREEO), Chabahar, Iran
4
.Persian Gulf Mollusks Research Station, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bandar‐e‐Lengeh, Iran
10.22124/japb.2024.28244.1551
Abstract
This research was conducted with the aim of extracting, identifying, and evaluating the antibacterial activity of the polysaccharide from the halophilic cyanobacterium Cyanothece sp. This cyanobacterium was cultured under laboratory conditions, and its biomass was collected. The polysaccharide extraction process was optimized by examining the effects of temperature (40, 60, and 80 C°) and extraction time (60, 120, and 180 minutes). After initial extraction, the raw polysaccharide was purified using ion exchange chromatography and gel filtration methods. The physicochemical properties of the extracted polysaccharide, including homogeneity, molecular weight, and monosaccharide profile, were analyzed. The antibacterial activity of the polysaccharide against Escherichia coli and Staphylococcus aureus bacteria was evaluated in vitro using the disk diffusion method. The results of this research showed that the highest yield of polysaccharide extraction was achieved at a temperature of 80 C° and for a duration of 180 minutes. The extracted polysaccharide had a molecular weight of 103 kilodaltons. The profile of monomeric units of rhamnose, fucose, xylose, mannose, glucose, galactose, glucuronic acid, and galacturonic acid was 7:1, 0.8, 0.1, 2:3, 1:6, 4:5, 5, and 6% in molar ratios, respectively. The results showed that the extracted polysaccharide significantly (p < 0.05) inhibited the growth of E. coli and S. aureus bacteria. Based on the results of this research, the polysaccharide derived from the microalga Cyanothece sp. has suitable antibacterial activity and can be used as a potential compound for pharmaceutical and health applications.
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