The effect of polystyrene nanoplastics on the growth performance, survival rate, liver enzyme activity and the expression of the IL1 and IL8 genes in zebrafish (Danio rerio)

Document Type : Research Paper

Authors

1 Associate Professor in Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran

2 Assistance Professor in Department of Clinical Science, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

3 Scientific Member in Department of Mesenchymal Stem Cell, Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran

4 Associate Professor in Department of Environmental Sciences, Faculty of Natural Resources, University of Zabol, Zabol, Iran

5 - Associate Professor in Department of Aquaculture, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

6 Postdoc in Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran. 7- Professor in Department of Chemistry, Faculty of Science, University of Zabol, Zabol, Iran

7 Professor in Department of Chemistry, Faculty of Science, University of Zabol, Zabol, Iran

8 Ph.D. in Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

10.22124/japb.2023.24905.1504

Abstract

There are widespread concerns about the potential toxic effects of microplastics as emerging pollutants. The current research investigated the impact of polystyrene nanoplastics on the growth performance, survival rate, liver enzymes activity and the immune gene expression of IL1 and IL8 genes in zebrafish (Danio rerio). Fish were divided into four treatments and fed with diets containing 0 (control), 100 (treatment 1), 500 (treatment 2) and 1000 (treatment 3) mg/kg diet for one month. The present study results showed that weight gain (WG) and specific growth rate (SGR) were affected by the experimental treatments, and with increasing concentration of polystyrene nanoplastic, they decreased significantly (P<0.05). The best food conversion ratio (FCR) was obtained in control and treatment 1 (P<0.05). No mortality was observed through different treatments. The highest activity of alanine aminotransferase) ALT(, alkaline phosphatase) ALP(, and aspartate aminotransferase )AST( was observed in treatment 3 (P<0.05). The highest level of IL1 and IL8 gene expression were measured in treatment 3 and the lowest level was seen in control (P<0.05). In conclusion, the results of this research showed that a diet containing polystyrene nanoplastics had a negative effect on the growth (WG and SGR) and health indicators of the fish and the greatest disturbance in the growth and health of fish were observed in high concentrations.

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References

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Aquatic Physiology and Biotechnology
Volume 12, Issue 2
October 2024
Pages 1-21

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