Physiological changes in blood of common carp (Cyprinus carpio) kept with different densities in biofloc system with acute hypoxia stress

Document Type : Research Paper

Authors

1 M.Sc. in Fisheries, Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

2 Associate Professor in Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

3 Assistant Professor in Department of Biology, Faculty of Basic Sciences and Engineering, Gonbad Kavous University, Gonbad Kavous, Iran

10.22124/japb.2023.25520.1513

Abstract

Common carp (Cyprinus carpio) with densities of 6, 12, 24 and 48kg/m3 (D1, D2, D3 and D4, respectively) were stocked in biofloc environment with acute hypoxia stress in order to investigate the physiological response of fish. At the first stage, the fish were kept in biofloc conditions for 28 days, and then in the second stage of the experiment, the fish were separately divided in 12 tanks with a water volume of 25 liters (4 treatments × 3 replicates) with the same biological conditions, and the only variable was the stocking density of fish. Increasing stocking density in D4 treatment significantly increased WBC, RBC, hemoglobin and hematocrit, but decreased lymphocytes and liver enzymes (P<0.05). Stocking density significantly increased total Immunoglobulin, lysozyme and ACH50 were observed in D1and D2 treatments (6 and 12k/m3) (P<0.05). The stress index had a significant increase in D3 and D4 treatments (P<0.05). Stocking density in D1 and D2 treatments significantly increased superoxide dismutase enzyme and decreased malondialdehyde enzyme (P<0.05). In general, the storage density between 6 and 12kg/m3 is recommended to control the hypoxia stress in the biofloc environment, so that the problem can be solved within one to two hours.

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Main Subjects


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