Effects of different dietary levels of protein hydrolysate in diet on antioxidant activities and digestive enzymes of cultured Beluga (Huso huso) Juveniles

Hosseinpour Zelaty, Ali; Sohrabi Langaroudi, Tooraj; Sayyed Hassani, Mirhamed; Monsef Shokri, Maryam

doi:10.22124/japb.2024.27300.1538

Effects of different dietary levels of protein hydrolysate in diet on antioxidant activities and digestive enzymes of cultured Beluga (Huso huso) Juveniles

Document Type : Research Paper

Authors

¹ Iranian Fisheries Science Research Institute

² International Sturgeon research Institute, rasht, IRAN

³ International Sturgeon research Institute

⁴ International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.

10.22124/japb.2024.27300.1538

Abstract

The objective of this study was to investigate the effect of replacing fishmeal with fish protein hydrolysate (FPH) some oxidative stress indicies and digestive enzyme activities in beluga (Huso huso) juveniles. A total of 750 beluga juveniles with an initial weight of 3 ± 0.5 g were randomly distributed into five experimental groups: a control group (without FPH), three treatments with different levels of FPH replacing fishmeal (treatments 1, 2, and 3 at 2.75, 5.5 and 8.25%, respectively), and a positive control group (commercial feed). The fish were cultivated for a period of 48 days in a system consisting of 15 tanks, each with a capacity of 500 liters and subjected to identical environmental conditions. The results demonstrated no significant differences were observed in enzyme activities of Catalase and superoxide dismutase and level of malondialdehyde activities among the experimental groups (P > 0.05). For digestive enzymes trypsin, pepsin, amylase, and lipase, no significant statistical differences (P > 0.05) were observed in any of the four stages (5, 10, 20 and 35 days after stocking) of enzyme activity assessment or in any of the types of enzymes studied. Overall, the results of this study suggest that replacing fishmeal with FPH up to 8.25% in the diet of beluga juveniles has no significant effect on the activity of the antioxidant and digestive systems of these fish.

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