Document Type : Research Paper
Authors
1
Marine Sciences Faculty, Fisheries Group, Chabahar Maritime University
2
2. Agricultural Research Educations and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Boss of Chabahar Offshore Fisheries Research Center, Chabahar, Iran
3
MS.c Chabahar Maritime University, Deparment of Marine Sciences, Fisheries groups
4
3. MS.c Chabahar Maritime University, Deparment of Marine Sciences, Fisheries groups
10.22124/japb.2025.28719.1555
Abstract
In recent decades, the use of appropriate and high-quality diets has been a crucial factor in the development of the shrimp industry, its growth, and the maintenance of its health. This study investigates the effect of a combined aqueous extract of brown macroalgae and the bacterium Bacillus subtilis (a single-cell prebiotic) on the hemolymph indices and non-specific immunity of the western whiteleg shrimp (Litopenaeus vannamei). A total of 2400 post-larval shrimp, each averaging 87.0±29 milligrams, were randomly assigned to eight groups of 100 individuals each in three replicates in tanks. The control group received a diet without the combined macroalgae extract (MPE) and single cell probiotic (P), while the other groups were fed diets containing varying amounts of the macroalgae extract and probiotic: 15 g of MPE kg-1 diet, 1 g of single-cell probiotic (P1 100 g-1 of diet, 2 grams (P2) and 3 g (P3), and combinations of the macroalgae extract with the probiotic at concentrations of 1 g (P1+MPE), 2 g (P2+MPE) and 3 g (P3+MPE) per 100 g of diet, for a period of 60 days. The results showed that the highest total hemocyte count was observed in the groups fed with 2P+MPE and 3P+MPE, at 15.36±0.35 and 15.10±0.36 (×106/mL), respectively, which were significantly different from the other experimental groups and the control group (P<0.05). The simultaneous use of an aqueous extract of macroalgae with different concentrations of single-cell probiotics significantly increased the number of granular cells, semi-granular cells, and hyaline cells compared to the control group and other experimental groups. The highest counts of these cells were found in the groups fed with 2P+MPE and 3P+MPE, showing a significant difference from the other experimental groups (P<0.05). However, no significant difference was observed between the groups fed with 2P+MPE and 3P+MPE (P>0.05).The group fed with 2P+MPE had the highest protein level (111.47±9.20 mg/mL) and the lowest glucose level (9.0±0.91 mg/dL), which were significantly different from the control group and other experimental groups (P<0.05). Overall, the use of a combined extract of brown seaweed with a concentration of 2g /100g diet of probiotic supplement resulted in the improvement of blood parameters and the non-specific immune system in the western white shrimp
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