The effect of immobilization technique and salinity (sodium chloride) stress on growth indices, carotenoid and carbohydrate accumulation in microalga Dunaliella salina

Document Type : Research Paper

Authors

1 Assistant Professor in Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran

2 M.Sc. in Biochemistry, Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran

10.22124/japb.2024.25973.1520

Abstract

Microalgae are used to produce valuable natural compounds. Low yield and high cost limit the commercial potential of microalgae. Recently, the techniques of immobilization and increasing production efficiency have been noticed. In the present study, the effect of salt concentration, stabilization and growth period on growth, biomass, carbohydrate, carotenoid, chlorophyll a and b in Dunaliella salina was evaluated. The results showed that the growth of microalgae in free conditions was more than in stabilization condition. At salinity of 150 and 300g/L algae growth was significantly reduced. The maximum biomass was measured at 75g/L salinity and free condition. Carotenoid in salinity of 75g/L and unstabilized condition was more than other treatments. Carbohydrate in salinity of 30g/L was significantly higher than other treatments. In the concentration of 150g/L, carbohydrate was significantly higher in stabilization condition. It seems that the composition of the substrate as well as the method of application of stabilization is an effective factor. Salt stress is a simple method to induce carotenoid synthesis. Research in the field of optimization of the material used as well as the method of applying stabilization to increase the production efficiency of valuable compounds seems necessary.

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References

Ahmad A., Banat F., Alsafar H. and Hasan S.W. 2023. Recent breakthroughs in integrated bio-molecular and biotechnological approaches for enhanced lipid and carotenoid production from microalgae. Phytochemistry Reviews, 22(4): 993–1013. doi: 10.1007/s11101-022-09804-5
Arnon A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112–121.
Ben-Amotz A., Polle J.E.W. and Subba Rao D.V. 2009. The Alga Dunaliella: Biodiversity, Physiology, Genomics and Biotechnology. CRC Press, USA. 575P.
Calderon N.D.G., Bayona K.C.D. and Garces L.A. 2018. Immobilization of the green microalga Botryococcus braunii in polyester wadding: Effect on biomass, fatty acids, and exopolysaccharide production. Biocatalysis and Agricultural Biotechnology, 14: 80–87. doi: 10.1016/j.bcab.2018.02.006
Gallego-Cartagena E., Castillo-Ramirez M. and Martinez-Burgos W. 2019. Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina. Saudi Journal of Biological Sciences, 26(7): 1325–1330. doi: 10.1016/j.sjbs.2019.07.010
Gomez P.I., Barriga A., Cifuentes A.S. and Gonzalez M.A. 2003. Effect of salinity on the quantity and quality of carotenoids accumulated by Dunaliella salina (strain CONC-007) and Dunaliella bardawil (strain ATCC 30861) chlorophyta. Biological Research, 36(2): 185–192. doi: 10.4067/S0716-97602003000200008
Hashemi A., Pajoum Shariati F., Delavari Amrei H. and Heydari Nasab A. 2021. The effect of instantaneous and slow-release salt stress methods on beta-carotene production within Dunaliella salina cells. Iranian Journal of Chemistry and Chemical Engineering, 40(5): 1642–1652. doi: 10.30492/IJCCE.2020.107691.3581
Hosseini Tafreshi A. and Shariati M. 2009. Dunaliella biotechnology: Methods and applications. Journal of Applied Microbiology, 107(1): 14–35. doi: 10.1111/j.1365-2672.20 09.04153.x
Jimenez C.A.R.L.O.S. and Pick U. 1994. Differential stereoisomer compositions of β, β-carotene in thylakoids and in pigment globules in Dunaliella. Journal of Plant Physiology, 143(3): 257–263. doi: 10.1016/S0176-1617(11)81628-7
Kaparapu J. and Geddada M.N.R. 2020. Commercial applications of algae in the field of biotechnology. P: 229–246. In: Arumugam M., Kathiresan S. and Nagaraj S. (Eds.). Applied Algal Biotechnology. Nova Science Publishers, India.
Lamers P.P., Janssen M., De Vos R.C., Bino R.J. and Wijffels R.H. 2008. Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. Trends in Biotechnology, 26(11): 631–638.
Mahdian Y. 2022. The effect of CO2 pump on the improvement of carotenoid production in Dunaliella salina microalgae (In Persian). M.Sc. Thesis, University of Kurdistan, Iran. 101P.
Mathimani T., Kumar T.S., Chandrasekar M., Uma L. and Prabaharan D. 2017. Assessment of fuel properties, engine performance and emission characteristics of outdoor grown marine Chlorella vulgaris BDUG 91771 biodiesel. Renewable Energy, 105: 637–646. doi: 10.1016/j.renene.2016.12.090
Mallick N. 2020. Immobilization of microalgae. P: 373–391. In: Guisan J.M. (Ed.). Immobilization of Enzymes and Cells: Methods in Biotechnology. Humana Press, USA.
Maleki L., Malek M. and Palm H.W. 2015. Four new species of Acanthobothrium Van Beneden, 1850 (Cestoda: Onchoproteo-cephalidea) from the guitarfish, Rhynchobatus cf. djiddensis (Elasmobranchii: Rhynchobatidae), from the Persian Gulf and Gulf of Oman. Folia Parasitologica, 62(12): 11–15. doi: 10.14411/fp.20 15.012
Moreno-Garrido I. 2013. Microalgal immobilization methods. P: 327–347. In: Guisan J.M. (Ed.). Immobilization of Enzymes and Cells: Methods in Biotechnology. Humana Press, USA. doi: 10.1007/ 978-1-62703-550-7
Mishra A., Mandoli A. and Jha B. 2008. Physiological characteriz-ation and stress-induced metabolic responses of Dunaliella salina isolated from salt pan. Journal of Industrial Microbiology and Biotechnology, 35(10): 1093–1101. doi: 10.1007/s10295-008-0387-9
Mohammadi K.E. and Arashrad F. 2016. Effect of different salinity levels on β-carotene production by Dunaliella sp. isolates from the Maharlu Lake, Iran. Medical Laboratory Journal, 10(5): 58–68. doi: 10.18869/acadpub.mlj.10.5.58
Nguyen A., Tran D., Ho M., Louime C., Tran H. and Tran D. 2016. High light stress regimen on Dunaliella salina strains for carotenoids induction. Integrative Food, Nutrition and Metabolism, 3: 347–350. doi: 10.15761/IFNM.10001 58
Olfati N. 2022. The effect of immobilization technique and salinity stress on carotenoid production in Dunaliella salina microalgae (In Persian). M.Sc. Thesis, University of Kurdistan, Iran. 110P.
Pasqualetti M., Bernini R., Carletti L., Crisante F. and Tempesta S. 2011. Salinity and nitrate concentration on the growth and carotenoids accumulation in a strain of Dunaliella salina (Chlorophyta) cultivated under laboratory conditions. Transitional Waters Bulletin, 4(2): 94–104. doi: 10.1285/i1825229Xv4n2p42
Sadeghi S.F. 2021. Effect of immobilization techniques and salinity stress on growth indicator and carbohydrate production in micro algae Dunaliella salina (In Persian). M.Sc. Thesis, University of Kurdistan, Iran. 98P.
Tran D., Doan N., Louime C., Giordano M. and Portilla S. 2014. Growth, antioxidant capacity and total carotene of Dunaliella salina DCCBC15 in a low cost enriched natural seawater medium. World Journal of Microbiology and Biotechnology, 30: 317–322. doi: 10.1007/s11274-013-1413-2
Wu M., Zhu R., Lu J., Lei A., Zhu H., Hu Z. and Wang J. 2020. Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6. Annals of Microbiology, 70(1): 1–9. doi: 10.1186/s13213-020-01588-3
Ye Z.W., Jiang J.G. and Wu G.H. 2008. Biosynthesis and regulation of carotenoids in Dunaliella: Progresses and prospects. Biotechnology Advances, 26(4): 352–360. doi: 10.1016/j.biotechadv. 2008.03.004
Zarandi-Miandoab L., Hejazi M.A., Bagherieh-Najjar M.B. and Chaparzadeh N. 2019. Optimiz-ation of the four most effective factors on β-carotene production by Dunaliella salina using response surface methodology. Iranian Journal of Pharmaceutical Research, 18(3): 1566–1579. doi: 10.22037/ijpr.2019.1100752
 
Aquatic Physiology and Biotechnology
Volume 12, Issue 3
February 2025
Pages 23-46

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