Comparison of spring and autumn migratory populations of Caspian white fish (Rutilus frisii) to Anzali Wetland using Cyt-b gene sequencing method

Gorouhi, Danial; Mousavi Sabet, Seyed Hamed

doi:10.22124/japb.2025.29480.1560

Comparison of spring and autumn migratory populations of Caspian white fish (Rutilus frisii) to Anzali Wetland using Cyt-b gene sequencing method

Articles in Press

Document Type : Research Paper

Authors

¹ Department of Fisheries, Faculty of Natural Resources, Gilan University, Rasht, Iran

² Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran.

10.22124/japb.2025.29480.1560

Abstract

In this study, the structure of spring and autumn Caspian white fish (Rutilus frisii) populations migrating to Anzali Wetland was studied using the Cyt-b gene sequencing method. The fish samples (60 samples from each population) were caught in 2019 and 2020. Polymerase chain reaction (PCR) and sequencing of the samples were performed after DNA extraction from the soft tissue of the pectoral fin. The results of molecular studies showed that cytosine and guanine had the highest and lowest average nucleotide composition values in the two populations, respectively. Calculation of the transition-to-crossover substitution rate indicated a higher rate of transition-to-crossover mutations, comprising 67.22% of the mutations. Based on the one-way variance test, a significant difference was observed in terms of genetics between the two populations of spring and autumn fish (P<0.05). The genetic distance between the two populations was 0.388, and the genetic similarity was 0.612. In general, it can be said that the Cyt-b gene has shown the difference between the two spring and autumn forms well. However, the basis of the molecular study of the populations in question, despite the time gap, showed significant genetic similarities at the species level. And these populations cannot be considered to have a significant difference in terms of genetics.

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