Isolation of symbiotic bacteria of Entromorpha flexuosa algae and investigation of their characteristics

SHAKOURI, ARASH; Sanchooli, Hamed; Naseri, Fatemeh

doi:10.22124/japb.2025.30752.1574

Isolation of symbiotic bacteria of Entromorpha flexuosa algae and investigation of their characteristics

Articles in Press

Document Type : Research Paper

Authors

¹ CHABAHAR MARITIME UNIVERSITY ( CMU )

² Chabahar University of Maritime and Marine Sciences, Chabahar, Iran

³ Shiraz University, Shiraz, Iran

10.22124/japb.2025.30752.1574

Abstract

In the marine environment, microorganisms colonize all surfaces, including various marine organisms such as macroalgae. These microorganisms associated with marine macroalgae represent a significant source of biologically active metabolites. Biofouling remains a persistent problem in the marine sector, necessitating effective control and cleaning strategies. The search for antifouling bacteria is essential to controlling biofouling, particularly given the environmental pollution associated with the use of toxic chemicals. In this study, four symbiotic bacteria were isolated and purified from Enteromorpha flexuosa macroalgae collected from the Oman Sea. Molecular identification of these bacteria, based on 16S rRNA gene sequence analysis, identified them as Bacillus zhangzhouensis strain Chabahar 1, Virgibacillus dokdonensis strain Chabahar 2, Micrococcus endophyticus strain Chabahar 3, and Bacillus subtilis strain Chabahar 4. These sequences were deposited in GenBank under the accession numbers OL472895, OL514166, OL514165, and OL514183, respectively. A phylogenetic tree was constructed to illustrate the kinship relationships among these bacteria. Ethanol extracts were prepared from these bacteria and screened for activity using the well diffusion method. However, these extracts did not exhibit any growth inhibitory effect.

Keywords