In vivo evaluation of the protective effect of Lactobacillus corvatus strain af1 isolated from fish farming ponds against lead and cadmium toxicity in intestinal and liver tissue of  Caspian Sea kutum (Rutillus frisii) fry

Fahim, Afshin; Anvari, Masoomeh; Valipour, Alireza; Safabakhsh, Mohammad Reza

doi:10.22124/japb.2025.31993.1579

In vivo evaluation of the protective effect of Lactobacillus corvatus strain af1 isolated from fish farming ponds against lead and cadmium toxicity in intestinal and liver tissue of Caspian Sea kutum (Rutillus frisii) fry

Articles in Press

Document Type : Research Paper

Authors

¹ Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran

² Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Guilan, Iran

10.22124/japb.2025.31993.1579

Abstract

In this research, the probiotic effects of Lactobacillus corvatus bacteria isolated from fish ponds were investigated for histopathological studies. For this purpose, white fish fry with an approximate weight of 8.05 ± 1.58 grams were randomly divided into six groups. The control group, the group with only Lactobacillus curvatus (10 8 Cfu/g), the group exposed to lead (12 mg), the group exposed to cadmium (4 mg), the group + Lactobacillus corvatus (10 8 Cfu/g with lead) and the cadmium group + Lactobacillus curvatus (10 8 Cfu/g with cadmium). Each treatment was done in three replicates. After adapting to the environmental conditions for 14 days, the treatments were kept for 56 days. After the mentioned period, the anesthetized fishes, the intestines and livers of the fishes were isolated, stored in Boen's solution and transferred to a specialized laboratory for histopathology tests. The results of microscopic tests showed that in the treatment containing lead and cadmium, hyperemia (low), the presence of blood cells in the lamina propria (lp), necrosis of enterocyte cells and microvilli and cell vacuoles were observed in the intestines of juvenile fish. Histopathological examination of liver tissue showed hyperemia and bleeding (high), cell necrosis (high) and cell vacuolation (low). After examining the liver and intestinal tissues of fishes exposed to heavy metals lead and cadmium compared to the treatments containing probiotics, the amount of tissue repair was observed in the treatment containing probiotics. Therefore, according to the results, a favorable effect on the liver tissue was observed at this stage.

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