Isolation of symbiotic bacteria from the alga Enteromorpha flexuosa and investigation of their antibacterial properties

Document Type : Research Paper

Authors

1 M.Sc. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

2 Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

3 M.Sc. in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

10.22124/japb.2025.30752.1574

Abstract

In the marine environment, all surfaces, including a variety of marine organisms such as marine macroalgae, are colonized by microorganisms. Microorganisms associated with marine macroalgae offer tremendous potential for biologically active metabolites. Fouling is a persistent problem in the marine sector that requires control and cleaning processes. The search for anti-fouling bacteria is essential for controlling fouling due to environmental pollution associated with the use of toxic chemicals. Four symbiotic bacteria were isolated and purified from Enteromorpha flexuosa macroalgae collected from the Oman Sea. Molecular identification of these bacteria based on 16 S rRNA gene sequences showed bacteria Bacillus zhangzhouensis strain Chabahar 1, Virgibacillus dokdonensis strain Chabahar 2, Micrococcus endophyticus strain Chabahar 3 and Bacillus subtilis strain Chabahar 4. These sequences were registered with the Gene Bank under the access numbers OL472895, OL514166, OL514165 and OL514183, respectively. Ethanol extract was extracted from these bacteria and screened by well diffusion method. These bacteria showed no growth inhibitory effect on each other.

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References

Dalton H., Poulsen L., Halasz P., Angles M., Goodman A. and Marshall K. 1994. Substratum-induced morphological changes in a marine bacterium and their relevance to biofilm structure. Journal of Bacteriology, 176(22): 6900–6906. doi: 10.1128/jb.176.22. 6900-6906.1994
Hou Q., Bai X., Li W., Gao X., Zhang F., Sun Z. and Zhang H. 2018. Design of primers for evaluation of lactic acid bacteria populations in complex biological samples. Frontiers in Microbiology, 2045: 1–10. doi: 10.3389/fmicb.2018.02045
JanakiDevi V., YokeshBabu M., Umarani R. and Kumaraguru A. 2013. Antagonistic activity of sea-weed associated bacteria against human pathogens. International Journal of Current Microbiology and Applied Sciences, 2(12): 140–147. doi: 10.20546/ijcmas
Karthick P. and Mohanraju R. 2018. Antimicrobial potential of epiphytic bacteria associated with seaweeds of Little Andaman, India. Frontiers in Microbiology, 9(611): 1–11. doi: 10.3389/fmicb.2018.00611
Marhaeni B., Radjasa O., Khoeri M., Sabdono A., Bengen D. and Sudoyo H. 2011. Antifouling activity of bacterial symbionts of seagrasses against marine biofilm-forming bacteria. Journal of Environmental Protection, 2: 1–5. doi: 10.4236/jep.2011.29143
Mondol M., Shin H. and Islam M. 2013. Diversity of secondary metabolites from marine Bacillus species: Chemistry and biological activity. Marine Drugs, 11(8): 2846–2872. doi: 10.3390/md110828 46
Rostami H., Hamedi H. and Yolmeh M. 2016. Some biological activities of pigments extracted from Micrococcus roseus (PTCC 1411) and Rhodotorula glutinis (PTCC 5257). International Journal of Immunopathology and Pharmacology, 29(4): 684–695. doi: 10.1177/0394632016673846
Senthilkumar P., Durga Devi V., Minhajdeen A., Saranya R., Sree Jaya S. and Sudha S. 2014. Antibacterial properties of Enteromorpha flexuosa (Wulfen) from the Gulf of Mannar-Southeast Coast of India. American Journal of Ethnomedicine, 1(1): 1–6. doi: 10.33714/masteb.640614
Shakouri A., Reisi J. and Zadabbas Shahabadi H. 2018. Biological activity of marine algal symbiotic bacteria. The first National Conference on Recent Advances in Engineering and Modern Sciences, Iran. 3427P. [In Persian].
Susilowati R., Sabdono A. and Widowati I. 2015. Isolation and characterization of bacteria associated with brown algae Sargassum spp. from Panjang Island and their antibacterial activities. Procedia Environmental Sciences, 23: 240–246. doi: 10.10 16/j.proenv.2015.01.036
Tamura K., Peterson D., Peterson N., Stecher G., Nei M. and Kumar S. 2011. MEGA 5: Molecular evolutionary genetics analysis using maximum like-lihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 1–9. doi: 10.1093/molbev/ msr121
Thompson J., Higgins D. and Gibson T. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 1–8. doi: 10.1093/nar/22.22.4673
Wang X., Huang Y., Sheng Y., Su P., Qiu Y., Ke C. and Feng D. 2017. Antifouling activity towards mussel by small-molecule compounds from a strain of Vibrio alginolyticus bacterium associated with sea anemone Haliplanella sp. Journal of Microbiology and Biotechnology, 27(3): 460–470. doi: 10.4014/jmb.1607.07068
Aquatic Physiology and Biotechnology
Volume 14, Issue 1
September 2026
Pages 1-13

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