In vivo evaluation of the protective effect of Lactobacillus curvatus strain af1 isolated from fish farming ponds against lead and cadmium toxicity in the intestinal and liver tissues of Caspian Sea kutum ( Rutilus frisii)

Document Type : Research Paper

Authors

1 Ph.D. Student in Microbiology, Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Associate Professor in Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

3 Associate Professor in Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Guilan, Iran

4 Scientific Member in Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

10.22124/japb.2025.31993.1579

Abstract

In this research, the probiotic effects of Lactobacillus curvatus bacteria isolated from fish ponds were investigated for histopathological studies. Kutum fingerling with an approximate weight of 8.05±1.58g were randomly divided into six groups including the control group, the group treated with L. curvatus (108CFU/g), the group treated with lead (12mg/kg), the group treated with cadmium (4mg/kg), the group treated with lead (12mg/kg) + L. curvatus (108CFU/g) and the group treated with cadmium + L. curvatus (108CFU/g); and experimental materials were added to their diets. Each treatment was done in three replicates. Fish were maintained under these treatments for 56 days. Then, fish intestine and liver were sampled for histopathological examination. The results showed that in the treatment containing lead and cadmium, hyperemia (low), the presence of blood cells in the lamina propria, necrosis of enterocyte cells and microvilli and cell vacuoles were observed in the intestines of juvenile fish. Histopathological examination of liver tissue showed hyperemia and bleeding (high), cell necrosis (high) and cell vacuolation (low). After examining the liver and intestine tissues of fishes exposed to heavy metals lead and cadmium compared to the treatments containing probiotics, the tissue regeneration was observed in the treatments containing probiotic. Therefore, according to the results, a favorable effect on the liver tissue was observed at this stage.

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Main Subjects

References

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Aquatic Physiology and Biotechnology
Volume 14, Issue 1
September 2026
Pages 15-45

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