Toxic effects of titanium dioxide nanoparticles (TiO2 NPs) on hematological indices in common carp (Cyprinus carpio) and roach (Rutilus rutilus)

Document Type : Research Paper


1 University of Gorgan

2 Gorgan University of Agriculture and Natural Resources


Despite the expansion of nanotechnology in various industries, a proper evaluation of lethal concentration of nanoparticles on fishes has not been investigated. In present study, the toxicity of titanium nanoparticles was studied in juveniles of common carp and roach. For this study, 105 pieces of roach and common carp with total length of 5.46±0.57cm and 7.36±0.30 cm, respectively, were placed in 60L glass water tanks. According to the LC50 test, toxicity of titanium for common carp and roach were obtained as 12523.44±0.47 and 8693.37±1.96 ppm respectively. After 7 days of exposure to sub-lethal concentrations of titanium dioxide, blood samples were taken. The haematological indices showed significant decrease in red blood cells, hemoglobin and hematocrit values (P<0.05) exposed to sub-lethal concentrations when compared to the control group. The results of this study showed that titanium dioxide nanoparticles can have a negative effect on blood parameters and reduced the amount of red blood cells and hemoglobin content of blood which caused fish death.