The effect of temperature changes and fetal bovine serum concentration on the growth of cultivated ovarian cells of Liza klunzingeri

Document Type : Research Paper

Authors

1 Ph.D. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

2 Scientific Member in Department of Biology, Payame Noor University, Tehran, Iran

3 Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, University of Mazandaran, Babolsar, Iran

4 Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

5 Assistant Professor in Molecular and Cellular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

The aim of this study was to evaluate the effect of fetal bovine serum (FBS) and temperature changes on the cultivated ovarian cells of Liza klunzingeri. For this purpose, 10 fish weighing 45 to 50 grams were caught and transferred to the laboratory. After disinfecting the body of the fish with 70% ethanol, the ovarian tissue was removed from the body and divided by scissors into small pieces. The ovarian cells were aspirated by using trypsin enzyme and were cultivated in the flasks containing L-15 medium under different temperature conditions (20, 25, 28, 30 and 32°C) and the serum concentrations of bovine embryos (0, 10, 15 and 20%). Cell count showed the maximum density of L. klunzingeri ovarian cells was observed in 15% FBS concentration, while temperature variations had no significant effect on cell proliferation. In conclusion, the most suitable conditions for the culture of ovarian cells in L. klunzingeri are the use of FBS 15% and temperatures above 20°C.

Keywords

References

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Aquatic Physiology and Biotechnology
Volume 5, Issue 4
March 2018
Pages 27-39

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