A survey on effect of different concentrations of Lactobacillus casei on serum antioxidative defense status and biochemical parameters of rainbow trout exposed to foodborne lead toxicity

Document Type : Research Paper

Authors

1 Associate Professor in Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Ph.D. in Aquatic Health, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Assistant Professor in Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Ph.D. Student in Veterinary Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 Department of clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

This study was aimed to investigate the effects of different concentrations of Lactobacillus casei on some serum biochemicals and antioxidant factors in rainbow trout exposed to foodborne lead toxicity. For this purpose, 375 juvenile fish (15±4.6g) were randomly divided into five groups in three replications. Group 1, 2 and 3 were respectively fed with a diet containing 5×106, 5×107 and 5×108 CFU/g Lactobacillus casei throughout the experiment. Group 4 (negative control) was fed with a basal diet (no probiotic and lead) during all experiment period. Group 5 (positive control) was fed with the basal diet for 45 days and was subsequently fed with diets containing 500 µg/kg lead nitrate similar to the probiotic groups for 21 days. Blood sampling was performed to measure the parameters on days of 0, 45, 52, 59, and 66. The results showed that LDH, cholesterol and triglyceride levels were significantly reduced in most probiotic treatments after probiotic consumption for 45 days compared to a negative control group (p < 0.05). After exposure to lead, serum ALP and LDH values in group 2 were significantly lower than group 5 (p < 0.05). In group 2, GSH enzyme levels significantly increased compared to group 5 on day 52 (p < 0.05). The findings of this study showed that supplementing the diet with Lactobacillus casei in rainbow trout can be effective in preventing the chronic effects of lead poisoning by improving the biochemical and antioxidant indices. It seems that level of 5×107 CFU/g of probiotic bacteria had an ameliorative effect on improving of the biomarkers.

Keywords

References

Alak G., Atamanalp M., Topal A., Arslan H., Kocaman E.M. and Oruc E. 2013. Effect of sub-lethal lead toxicity on the histopathological and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss). Fresenius Environmental Bulletin, 22: 733–738.
Al-Dohail M.A., Hashim R. and Aliyu-Paiko M. 2011. Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles. Aquaculture Research, 42(2): 196 –209.
Alizadeh Rudposhti M., Shenavar Masouleh A., Masoumzadeh M., Jalilpoor J., Bazari Moghaddam S., Yeganeh H. and Azizzadeh L. 2017. Effect of lactic Acid bacteria Enterococcus faecalis as a probiotic on blood biochemical and serum factors in Persian sturgeon (Acipenser persicus) fingerlings. Journal of Aquaculture Development, 11(1): 89–103.
Alves L., Glover C. and Wood C. 2006. Dietary Pb accumulation in juvenile freshwater rainbow trout (Oncorhynchus mykiss). Archives of Environmental Contamination and Toxicology, 51(4): 615.
Casillas E., Myers M. and Ames W.E. 1983. Relationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride. Aquatic Toxicology, 3(1): 61–78.
Chelladurai G., Felicitta J. and Nagarajan R. 2013. Protective effect of probiotic diets on haematobiochemical and histopathology changes of Mystus montanus (Jerdon 1849) against Aeromonas hydrophila. Journal of Coastal Life Medicine. 1(4): 259–264.
Dai W., Fu L., Du H., Jin C. and Xu Z. 2009. Changes in growth performance, metabolic enzyme activities, and content of Fe, Cu, and Zn in liver and kidney of tilapia (Oreochromis niloticus) exposed to dietary Pb. Biological Trace Element Research, 128(2): 176–183.
Das P., Khowala S. and Biswas S. 2016. In vitro probiotic characterization of Lactobacillus casei isolated from marine samples. LWT-Food Science and Technology, 73: 383–390.
Dawood M.A., Koshio S., Ishikawa M. and Yokoyama S. 2015. Interaction effects of dietary supplementation of heat-killed Lactobacillus plantarum and β-glucan on growth performance, digestibility and immune response of juvenile red sea bream, Pagrus major. Fish and Shellfish Immunology, 45(1): 33 –42.
Dawood M.A., Koshio S., Ishikawa M., Yokoyama S., El Basuini M.F., Hossain M.S., Nhu T.H., Dossou S. and Moss A.S. 2016. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. Fish and Shellfish Immunology, 49: 275–285.
Dutta H.M. and Haghighi A.Z. 1986. Methylmercuric chloride and serum cholesterol level in the bluegill (Lepomis macrochirus). Bulletin of Environmental Contamination and Toxicology, 36(1): 181–185.
Eisler R. 1988. Lead hazards to fish, wildlife, and invertebrates: A synoptic review, Patuxent Wildlife Research Center, Laurel, MD (USA).
Ellman G.L. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82(1): 70–77.
Farkas W.R., Stanawitz T. and Schneider M. 1978. Saturnine gout: Lead-induced formation of guanine crystals. Science. 199(4330): 786–787.
Firat O. and Kargin F. 2010. Individual and combined effects of heavy metals on serum biochemistry of Nile tilapia Oreochromis niloticus. Archives of Environmental Contamination and Toxicology, 58(1): 151–157.
Gerbino E., Carasi P., Tymczyszyn E.E. and Gomez-Zavaglia A. 2014. Removal of cadmium by Lactobacillus kefir as a protective tool against toxicity. Journal of Dairy Research, 81(3): 280–287.
Javed M., Ahmad M.I., Usmani N. and Ahmad M. 2017. Multiple biomarker responses (serum biochemistry, oxidative stress, genotoxicity and histopathology) in Channa punctatus exposed to heavy metal loaded waste water. Scientific Reports, 7(1): 1–11 (1675).
Jezierska B. and Witeska M. 2006. The metal uptake and accumulation in fish living in polluted waters. P: 107–114. In: Twardowska I., Allen H.E., Haggblom M.M. and Stefaniak S. (Eds.). Soil and water Pollution Monitoring, Protection and Remediation. Springer, Netherlands.
Johnson A.M., Rohlfs E.M. Silverman L.M. 1999. Protein. P: 477–540. In: Burtis C.A. and Ashwood E.R. (Eds.). Tietz Textbook of Clinical Chemistry. WB Saunders, Philadelphia.
Kalia K. and Flora S.J. 2005. Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. Journal of Occupational Health, 47(1): 1–21.
Koroluk M., Ivanova L. and Maiorova I. 1988. The method of definition of the activeness of catalase. Laboratorial Work (1): 16–19.
Latha M. and Pari L. 2003. Preventive effects of Cassia auriculata L. flowers on brain lipid peroxidation in rats treated with streptozotocin. Molecular and Cellular Biochemistry, 243(1-2): 23–28.
Martinez C., Nagae M., Zaia C. and Zaia D. 2004. Acute morphological and physiological effects of lead in the neotropical fish Prochilodus lineatus. Brazilian Journal of Biology, 64(4): 797–807.
Meldrum J.B. and Ko K.W. 2003. Effects of calcium disodium EDTA and meso-2, 3-dimercaptosuccinic acid on tissue concentrations of lead for use in treatment of calves with experimentally induced lead toxicosis. American Journal of Veterinary Research, 64(6): 672–676.
Mirmazloomi S., Shahsavani D. and Baghshani H. 2015. Studies on the protective effects of ascorbic acid and thiamine on lead-induced lipid and protein oxidation as well as enzymatic alterations in some tissues of Cyprinus carpio. Comparative Clinical Pathology, 24(5): 1231–1236.
Monachese M.A. 2012. Sequesteration of lead, cadmium and arsenic by Lactobacillus species and detoxication potential. The School of Graduate and Postdoctoral Studies, The University of Western Ontario London, Ontario, Cana, 154P.
Monteiro S.M., Mancera J.M., Fontainhas-Fernandes A. and Sousa M. 2005. Copper induced alterations of biochemical parameters in the gill and plasma of Oreochromis niloticus. Comparative Biochemistry and Physiology C, 141(4): 375–383.
Moss D. and Henderson A. 1999. Clinical enzymology. P: 617–677. In: Burtis C.A. and Ashwood F.R. (Eds.). Tietz Textbook of Clinical Chemistry. WB Saunders Company, Philadelphia.
Mrvcic J., Stanzer D., Solic E. and Stehlik-Tomas V. 2012. Interaction of lactic acid bacteria with metal ions: Opportunities for improving food safety and quality. World Journal of Microbiology and Biotechnology, 28(9): 2771–2782.
Nemcsok J. and Hughes G. 1988. The effect of copper sulphate on some biochemical parameters of rainbow trout. Environmental Pollution, 49(1): 77–85.
Paul N. and Sengupta M. 2013. Lead induced overactivation of phagocytes and variation in enzymatic and non-enzymatic antioxidant defenses in intestinal macrophages of Channa punctatus. Modern Research in Inflammation, 2: 28–35.
Peixoto F.P., Carrola J., Coimbra A., Fernandes C., Teixeira P., Coelho L., Conceicao I., Oliveira M.M. and Fontainhas-Fernandes A. 2013. Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela River, Portugal. Revista Internacional de Contaminacion Ambiental, 29: 29–38.
Planas M., Vazquez J., Marques J., Perez-L Gonzalez M.P. and Murado M. 2004. Enhancement of rotifer (Brachionus plicatilis) growth by using terrestrial lactic acid bacteria. Aquaculture, 240(1-4): 313–329.
Rifai N., Bachorik P.S. and Albers J.J. 1999. Lipids, Lipoproteins and Apolipoproteins. Tietz Textbook of Clinical Chemistry: WB Saunders Company, Philadelphia. 927P.
Sacks D.B. 1999. BFACP carbohydrates. P: 787–790. In: Burtis C.A. and Ashwood E.R. (Eds). Tietz Book of Clinical Chemistry. Saunders Company, Philadelphia. 1022P.
Salinas I., Abelli L., Bertoni F., Picchietti S., Roque A., Furones D., Cuesta A., Meseguer J. and Esteban M.A. 2008. Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (Sparus aurata L.). Fish and Shellfish Immunology, 25(1-2): 114–123.
Seyedi B., Heidary R. and Tukmechi A. 2013. Dietary effect of L. casei and L. paracasei as probiotic bacteria with Raftilose as peribiotic on the growth and liver enzymes in rat. Razi Journal of Medical Sciences, 20(107): 1–9.
Shen W.Y., Fu L.L., Li W.F. and Zhu Y.R. 2010. Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei). Aquaculture Research, 41(11): 1691–1698.
Son V.M., Chang C.C., Wu M.C., Guu Y.K., Chiu C.H. and Cheng W. 2009. Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish and Shellfish Immunology, 26(5): 691–698.
Tang L., Huang K., Xie J., Yu D., Sun L., Huang Q. and Bi Y. 2017. 1- Deoxynojirimycin from Bacillus subtilis improves antioxidant and antibacterial activities of juvenile Yoshitomi tilapia. Electronic Journal of Biotechnology, 30: 39–47.
Tewari H., Gill T.S. and Pant J. 1987. Impact of chronic lead poisoning on the hematological and biochemical profiles of a fish, Barbus conchonius (Ham). Bulletin of Environmental Contamination and Toxicology, 38(5): 748–752.
Thy H.T.T., Tri N.N., Quy O.M., Fotedar R., Kannika K., Unajak S. and Areechon N. 2017. Effects of the dietary supplementation of mixed probiotic spores of Bacillus amyloliquefaciens 54A, and Bacillus pumilus 47B on growth, innate immunity and stress responses of striped catfish (Pangasianodon hypophthalmus). Fish and Shellfish Immunology, 60: 391–399.
Toutou M.M., Soliman A.A.A., Farrag M.M.S. and Abouelwafa A.E. 2016. Effect of probiotic and synbiotic food supplementation on growth performance and healthy status of grass carp, Ctenopharyngodon idella (Valenciennes, 1844). International Journal of Ecotoxicology and Ecobiology, 1(3): 111–117.
Vaglio A. and Landriscina C. 1999. Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicology and Environmental Safety, 43(1): 111–116.
Velmurugan B., Selvanayagam M., Cengiz E.I. and Uysal E. 2008. Levels of transaminases, alkaline phosphatase, and protein in tissues of Clarias gariepienus fingerlings exposed to sublethal concentrations of cadmium chloride. Environmental Toxicology, 23(6): 672–678.
Vine N., Leukes W., Kaiser H., Daya S., Baxter J. and Hecht T. 2004. Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus. Journal of Fish Diseases, 27(6): 319–326.
Yu L., Zhai Q., Zhu J., Zhang C., Li T., Liu X., Zhao J., Zhang H., Tian F. and Chen W. 2017. Dietary Lactobacillus plantarum supplementation enhances growth performance and alleviates aluminum toxicity in tilapia. Ecotoxicology and Environmental Safety, 143: 307–314.
Zhai Q., Tian F., Zhao J., Zhang H., Narbad A. and Chen W. 2016. Oral administration of probiotics inhibits absorption of the heavy metal cadmium by protecting the intestinal barrier. Applied and Environmental Microbiology, 82(14): 4429–4440.
Aquatic Physiology and Biotechnology
Volume 8, Issue 1
June 2020
Pages 37-68

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