CYP1A gene sequencing and Study of gene expression pattern in different tissues of mudskipper (Periophthalmus waltoni) captured from natural habitat

Document Type : Research Paper


1 Assistant Professor in Biotechnology Department, Persian Gulf Research Center, University of Persian Gulf, Boushehr, Iran

2 Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

3 Professor in Department of Parasitology, Faculty of Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran


Cytochrome CYP1A is one of the main involved enzymes in the metabolism of the xenobiotic. Identifying the cytochrome P450 (CYP1A), responsible for the major metabolic pathways, can lead to understanding the metabolism pathway of organic materials and PAHs adaptation of sea fish. Results from cloning and identifying CYP1A gene in Periophthalmus waltoni showed CYP1A contained 1035 nucleotide from open reading frame sequenced. CYP1A basal gene expression was varied from 23, 22, 19, 17, 11, 11% for gill, kidney, heart, intestine, brain respectively. According to the results, liver organ showed the highest response of CYP1A gene expression like other fish species. But, the basic gene expression of CYP1A in other organs in comparison with liver organ was higher. Although basal expression pattern of genes depending on the species, high basal expression of gene CYP1A due to P. waltoni was exposure to PAH in natural conditions. Different CYP1A gene expression pattern in different organs showed ecophysiological condition of mudskipper. So, P. waltoni has the potential to be used as an exact biomarker for evaluation of organic pollutant of ecosystems especially PAHs.


Abdoli A. 2000. The Inland Water Fishes of Iran. Iranian Museum of Nature and Wildlife, Iran. 378P.
Agorreta A., San Mauro D., Schliewen U., Van Tassell J.L., Kovacic M., Zardoya R. and Ruber L. 2013. Molecular phylo-genetic of Gobioidei and phylo-genetic placement of European gobies. Molecular Phylogenetics and Evolution 69(3): 619–633.
AnsariA.A.,TrivediS.,SagguS. and Rehman H. 2014. Mudskipper: A biological indicator for environmental monitoring and assessment of coastal waters. Journal of Entomology and Zoology Studies, 2(6): 22–33.
Brammell B.F., McClain J.S., Oris J.T., Price D.J., Birge W.J. and Elskus A.A. 2010. CYP1A expression in caged rainbow trout discriminates among sites with various degrees of polychlorinated biphenyl contamination. Archives of Environmental Contamination and Toxicology, 58(3): 772–782.
Campbell P.M. and Devlin R.H. 1996. Expression of CYP1A1 in livers and gonads of Pacific salmon: Quantitation of mRNA levels by RT-PCR. Aquatic Toxicology, 34(1): 47–69.
Dorrington T., Zanette J., Zacchi F.L., Stegeman J.J. and Bainy A.C.D. 2012. Basal and 3-methylcholanthrene-induced expression of cytochrome P450 1a, 1b and 1c genes in the Brazilian guppy, Poecilia vivipara. Aquatic Toxicology, 124–125: 106–113.
Erdogan O., Ceyhun S.B., Ekinci D. and Aksakal E. 2011. Impact of deltamethrin exposure on mRNA expression levels of metallo-thionein A, B and cytochrome P450 1A in rainbow trout muscles. Gene, 484: 13–17.
Gao K., Brandt I., Goldstone J.V. and Jonsson M.E. 2011. Cytochrome P450 1a, 1b, and 1c Mrna induction patterns in three-spined stickleback exposed to a transient and a persistent inducer. Comparative Biochemistry and Physiology C, 154(1): 42–55.
Jonsson M.E., Gao K., Olsson J.A., Goldstone J.V. and Brandt I. 2010. Induction patterns of new Cyp1 genes in environmentally exposed rainbow trout. Aquatic Toxicology, 98(4): 311–321.
Jonsson M.E., Orrego R., Woodin B.R., Goldstone J.V. and Stegeman J.J. 2007. Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1a, 1b and 1c genes in zebrafish. Toxicology and Applied Pharmacology, 221(1): 29–41.
Jung J.H., Kim M., Yim U.H., Ha S.Y., An J.G., Won J.H., Han G.M., Kim N.S., Addison R.F. and Shim W.J. 2011. Biomarker responses in pelagic and benthic fish over 1 year following the Hebei Spirit oil spill (Taean, Korea). Marine Pollution Bulletin, 62(8): 1859–1866.
Lee Y.M., Williams T.D., Jung S.O. and Lee J.S. 2005. cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus. Marine Pollution Bulletin, 51: 769–775.
Livak K.J. and Schmittgen T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25(4): 402–408.
Murdy E.O. 1989. A taxonomic revision and cladistic analysis of the Oxudercinae gobies (Gobiidae: Oxudercinae). Records of the Australian Museum, 11: 1–93.
Oh S.M., Park K. and Chung K.H. 2006. Combination of in vitro Bioassays encompassing different mechanisms to determine the endocrine-disrupting effects of river water. Science of the Total Environment, 354(2–3): 252–264.
Rees C.B. and Li W. 2004. Develop-ment and application of a real-time quantitative PCR assay for determining CYP1A transcripts in three genera of salmonids. Aquatic Toxicology, 66(4): 357–368.
Rees C.B., Wu H. and Li W. 2005. Cloning of Cyp1a in Atlantic salmon (Salmo salar). Aquaculture, 246(1–4): 11–23.
Shirani M., Mirvaghefi A., Farahmand H. and Abdollahi M. 2012. Biomarker responses in mudskipper (Periophthalmus waltoni) from the coastal areas of the Persian Gulf with oil pollution. Environmental Toxicology and Pharmacology, 43: 705–713.
TamuraK.,StecherG.,PetersonD.,Filipski A. and Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729.
Thacker C.E. 2003. Molecular phylogeny of the gobioid fishes. Molecular Phylogenetics and Evolution, 26(3): 354–368.
Thompson J.D., Gibson T.J. and Higgins D.G. 2002. Multiple sequence alignment using ClustalW and ClustalX. P: 2.3.1–2.3.22. In: Roberts R.J. (Ed.). Current Protocols in Bioinformatics, John Wiley and Sons, Inc. USA.
Uno T., Ishizuka M. and Itakura T. 2012. Cytochrome P450 (CYP) in fish. Environmental Toxicology and Pharmacology, 34(1): 1–13.
Yengi L.G., Xiang Q., Pan J., Scatina J.A., Kao J., Ball S.E., Fruncillo R., Ferron G. and Roland Wolf C. 2003. Quantitation of cytochrome
P450 mRNA levels in human skin. Analytical Biochemistry, 316: 103–110.
Yuan L., Lv B., Zha J., Wang Z., Wang W., Li W. and Zhu L. 2013. New cytochrome P450 1B1, 1C1, 2Aa, 2Y3, and 2K genes from Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression and response of rare minnow CYP1s and CYP2s mRNA exposed to the AHR agonist benzo [a] pyrene. Chemosphere, 93: 209–216.
Zanette J., Jenny M.J., Goldstone J.V., Woodin B.R., Watka L.A., Bainy A.C.D. and Stegeman J.J. 2009. New cytochrome P450 1b1, 1c2 and 1d1 genes in the killifish Fundulus heteroclitus: Basal expression and response of five killifish Cyp1s to the Ahr agonist Pcb126. Aquatic Toxicology, 93(4): 234–243.
Zapata-Perez O., Gold-Bouchot G., Ortega A., Lopez T., Albores A. 2002. Effect of pyrene on hepatic cytochrome P450 1A (CYP1A) expression in Nile tilapia (Oreochromis niloticus). Archives of Environmental Contamination and Toxicology