تولید پروتئین هیدرولیز شده از ماهی کیلکا با روش هیدرولیز آنزیمی و سنجش خواص زیست‌فعالی آن

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فرآوری محصولات شیلاتی، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

2 استادیار گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران

3 دانشیار گروه پلیمرهای زیست‌سازگار، پژوهشگاه پلیمر و پتروشیمی ایران، تهران، ایران

چکیده

در مطالعه حاضر، ابتدا با استفاده از آنزیم آلکالاز از ماهی کیلکای معمولی (Clupeonella cultriventris)­ پروتئین هیدرولیز شده (FPH) به دست آمد. سپس فعالیت آنتی‌اکسیدانی پروتئین هیدرولیز شده ماهی  به وسیله آزمون­های قدرت مهارکنندگی رادیکال‌های آزاد DPPH و ABTS، قدرت کاهندگی آهن،  فعالیت شلاته کنندگی یون آهن و فعالیت آنتی‌اکسیدانی کل  در 5 غلظت متفاوت (1، 2، 3، 4 و 5 میلی‌گرم در میلی‌لیتر)، مورد ارزیابی قرارگرفت. نتایج بیان کننده آن بود که با افزایش غلظت پروتئین، خاصیت آنتی‌اکسیدانی FPH به طور معنی­داری افزایش ­یافت. بالاترین فعالیت مهارکنندگی رادیکال DPPH، قدرت کاهندگی آهن، شلاته کنندگی یون آهن و فعالیت آنتی‌اکسیدانی کل مربوط به غلظت 5 میلی­گرم بر میلی­لیتر بود. در مجموع می­توان گفت هیدرولیز آنزیمی ماهی کیلکا منجر به تولید پروتئین‌هایی با خواص آنتی‌اکسیدانی می‌شود که می­توانند بعد از تایید مطالعات کلینیکی به عنوان افزودنی مواد غذایی مورد استفاده قرار گیرند.

کلیدواژه‌ها


مطلبیع. وپرافکندهف. 1388. وضعیت ذخایرماهیان کیلکا در دریای خزر. موسسه

تحقیقات شیلات ایران. 32ص.

Aleman A., Gimenez B., Perez-Santin E., Gomez-Guillen M. and Montero P. 2011. Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate. Food Chemistry, 125(2): 334–341.

Brand-Williams W., Cuvelier M. and Berset C. 1995. Use of free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28(1): 25–30.

Cencic A. and Chingwaru W. 2010. The role of functional foods, nutraceuticals, and food supplements in intestinal health. Nutrients, 2(6): 611–625.

Chai T.T., Tong S.R., Law Y.C., Ismail N.I.N. and Wong F.C. 2015. Anti-oxidative, metal chelating and radical scavenging effects of protein hydrolysates from blue-spotted stingray. Tropical Journal of Pharmaceutical Research, 14(8): 1349–1355.

Chew Y.L., Omar M. and Khoo K.S. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT- Food Science and Technology, 41(6): 1067–1072.

Dey S.S. and Dora K.C. 2011. Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus). Journal of Food Science and Technology, 51(3): 449–457.

Dinis T.C.P., Maderia V.C.M. and Almeida M.L.M. 1994. Action of phenolic derivates as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315: 161–169.

Dong S.Y., Liu Z.Y., Zeng M.Y., Zhao Y.H., Guo Y.H. and Wang D.F. 2008.  Functional characterization of protein hydrolysates from silver carp (Hypophthalmichthys molitrix) meat by two kinds of proteases. Food Science, 29(12): 94–98.

Foh M.B.K., Amadou I., Foh B.M., Kamara M.T. and Xia W. 2010. Functionality and antioxidant properties of tilapia (Oreochromis niloticus) as influenced by the degree of hydrolysis. International Journal of Molecular Sciences, 11(4): 1851–1869.

Hasler C.M. 2002. Functional foods: Benefits, concerns and challenges-A position paper from the American Council on Science and Health. The Journal of Nutrition, 132(12): 3772–3781.

Hathwar S.C., Bijinu B., Rai A.K. and Narayan B. 2011. simultaneous recovery of lipids and proteins by enzymatic hydrolysis of fish industry waste using different commercial proteases. Applied Biochemistry and Biotechnology, 164(1): 115–124.

Huang X., Dai J., Fournier J., Ali A.M., Zhang Q. and Frenkel K. 2002. Ferrous ion autoxidation and its chelation in iron-loaded human liver HepG2 cells. Free Radical Biology and Medicine, 32(1): 84–92.

Je J.Y., Qian Z.J., Byun H.G. and Kim S.K. 2007. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochemistry, 42: 840–846.

JoH.Y.,JungW.K.andKimS.K. 2008. Purification and characterization of a novel anticoagulant peptide from marine echiuroid worm, Urechis unicinctus. Process Biochemistry, 43(2): 179–184.

Khiari Z. 2010. Functional and bioactive components from mackerel (Scomber scombrus) and blue whiting (Micromesistius poutassou) processing waste. Ph.D. Thesis, Dublin Institute of Technology, Ireland. 323P.

Khora S.S. 2013. Therapeutic benefits of ω-3 fatty acids from fish. International Journal of Drug Development and Research, 5(2): 55–65.

Khoshkhoo Z., Motalebi A., Khanipour A.A., Firozjaee H.K. and Mahdabi M.N.M. 2010. Study on changes of protein and lipid of fish protein concentrate (FPC) produced form kilkas in VP and MAP packages at light and darkness condition during six months. International Journal of Environmental Science and Development, 1(1): 101–106.

Ko J.Y., Lee J.H., Samarakoon K., Kim J.S. and Jeon Y.J. 2013. Purification and determination of two novel antioxidant peptides from flounder fish (Paralichthys olivaceus) using digestive proteases. Food and Chemical Toxicology, 52: 113–120.

Kumar N.S., Nazeer R.A. and Jaiganesh R. 2011. Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein. Peptides, 32(7): 1496–1501.

Leong L.P. and Shui G. 2000. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chemistry, 76(1): 69–75.

Li Y., Jiang B., Zhang T., Mu W. and Liu J. 2008. Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH). Food Chemistry, 106(2): 444–450.

Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. 1951. Protein measurement with the Folin phenol reagent.  Journal of Biological Chemistry, 193(1): 265–275.

Mendis E., Rajapakse N. and Kim S.K. 2005. Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysates. Journal of Agricultural and Food Chemistry, 53(3): 581–587.

Mishra K., Ojha H. and Chaudhury N.K. 2012. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chemistry, 130(4): 1036–1043.

Mohammadi A.,  Jafari S.M., Esfanjani A.F. and Akhavan S. 2016. Application of nano-encapsulated olive leaf extract in controlling the oxidative stability of soybean oil. Food Chemistry, 190: 513–519.

Mosquera M., Gimenez B., da Silva I.M., Boelter J.F., Montero P., Gomez-Guillen M.C. and Brandelli A. 2014. Nano-encapsulation of an active peptidic fraction from sea bream scales collagen. Food Chemistry, 156(2): 144–150.

Najafian L. and Babji A.S. 2012. A review of fish-derived antioxidant and antimicrobial peptides: Their production, assessment, and applications. Peptides, 33(1):178–185.

Nalinanon S., Benjakul S., Kishimura H. and Shahidi F. 2011. Functionalities and antioxidant properties of protein hydrolysates from the muscle of ornate threadfin bream treated with pepsin from skipjack tuna. Food Chemistry, 124(4): 1354–1362.

Ovissipour M., Rasco B., Shiroodi S.G., Modanlow M., Gholami S. and Nemati M. 2012. Antioxidant activity of protein hydrolysates from whole anchovy sprat (Clupeonella engrauliformis) prepared using endogenous enzymes and commercial proteases. Journal of the Science of Food and Agriculture, 93(7): 1718–1726.

Prieto P., Pineda M. and Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269(2): 337–341.

Raghavan S. and Kristinsson H.G. 2009. ACE-inhibitory activity of tilapia protein hydrolysates, Food Chemistry, 117(4): 582–588.

Saiga A., Tanabe S. and Nishimura T. 2003. Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. Journal of Agricultural and Food Chemistry, 51(12): 3661–3667.

Salampess J., Phillips M., Seneweera S. and Kailasapathy K. 2010. Release of antimicrobial peptides through bromelain hydrolysis of leatherjacket (Meuchenia sp.) insoluble proteins. Food Chemistry, 120(2): 556–560.

Shahidi F., Han X.Q. and Synowiecki J. 1995. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chemistry, 53(3): 285–293.

Sharma N., Singh N.K., Singh O.P., Pandey V. and Verma P.K. 2011. Oxidative stress and antioxidant status during transition period in dairy cows. Asian-Australasian Journal of Animal Sciences, 24(4): 479–484.

Skouta R., Dixon S.J.,  Wang J., Dunn D.E., Orman M., Shimada K., Rosenberg P.A., Lo D.C., Weinberg J.M. and Linkermann A. 2014. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. Journal of the American Chemical Society, 136(12): 4551–4556.

Suetsuna K. 2000. Antioxidant peptides from the protease digest of prawn (Penaeus japonicus) muscle. Marine Biotechnology, 2(1): 5–10.

Thiansilakul Y., Benjakul S. and Shahidi F. 2007. Antioxidative activity of protein hydrolysate from round scad muscle using Alcalase and Flavourzyme. Journal of Food Biochemistry, 31(2): 266–287.

Zamani A., Madani R. and Rezaei M. 2016. Antioxidative activity of protein hydrolysate from the muscle of common Kilka (Clupeonella cultriventris caspia) prepared using the purified trypsin from common Kilka intestine. Journal of Aquatic Food Product Technology, 26 (1): 2–16.

Zarenejad F. and Peighambardoust S. 2014. Effect of stabilization on functional components and fatty acid profile of wheat germ. Iranian Journal of Nutrition Sciences and Food Technology, 8(4): 93–100.