بررسی تاثیر سطوح مختلف باکتری Lactobacillus casei بر وضعیت دفاع آنتی‌اکسیدانی و برخی فراسنجه‌های بیوشیمیایی سرم خون ماهی قزل‌آلای ‌رنگین‌کمان پس از مصرف سرب در جیره غذایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 دکتری بهداشت آبزیان، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استادیار موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

4 دانشجوی دکتری عمومی دامپزشکی، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

هدف از مطالعه حاضر بررسی اثرات مصرف باکتری Lactobacillus casei روی برخی از فراسنجه­های بیوشیمیایی و آنتی‌اکسیدانی سرم خون ماهی قز‌ل‌آلای ‌رنگین‌کمان پس از مواجهه با فلز سرب در جیره است. برای این منظور، 375 قطعه ماهی (6/4±15 گرم) به طور تصادفی به پنج گروه تقسیم شدند (با سه تکرار). گروه یک، دو و سه به ترتیب با جیره‌های حاوی 106×5، 107×5 و CFU/g 108×5 پروبیوتیک تغذیه شدند. گروه چهارم (شاهد منفی) با جیره پایه تغذیه شد. گروه پنجم (شاهد مثبت) ابتدا به مدت 45 روز با جیره پایه و سپس به مدت 21 روز همراه با گروه‌های پروبیوتیکی با غذای حاوی نیترات سرب تغذیه شد. خون‌گیری در روزهای صفر، 45، 52، 59 و 66 صورت گرفت. طبق نتایج مقادیر LDH، کلسترول و تری‌گلیسرید در اغلب تیمارهای پروبیوتیکی پس از 45 روز نسبت به گروه شاهد منفی کاهش معنی‌داری داشت (05/0p <). پس از مواجهه با سرب مقادیر ALP و LDH سرم در گروه دو نسبت به پنج از کاهش معنی‌داری برخوردار بود (05/0p <). در گروه دو سطح آنزیم گلوتاتیون در روز 52 افزایش معنی‌داری نسبت به گروه پنج داشته است (05/0p <). بر اساس نتایج، استفاده از پروبیوتیک مذکور در ماهی قزل­آلای رنگین‌کمان می‌تواند با بهبود شاخص­های بیوشیمیایی و آنتی‌اکسیدانی ماهی در پیشگیری از مسمومیت با سرب موثر باشد. همچنین به نظر می­رسد که سطح CFU/g107×5 باکتری تاثیر بهتری در بهبود فراسنجه‌های مورد مطالعه داشت.

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