بررسی اثر ضدسرطانی نانوذرات نقره بیوسنتز شده توسط عصاره‌ جلبک سبز Spirogyra sp. بر رده سلولی سرطان پستان MDA-MB-231

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوشیمی، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

2 دکتری بیوشیمی، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

3 دانشیار گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

10.22124/japb.2023.23586.1489

چکیده

امروزه روش سبز با استفاده از عصاره­های گیاهی برای تولید نانوذرات مورد استفاده در صنعت، به دلیل سریع بودن واکنش، اقتصادی بودن و سازگار بودن با محیط زیست توجه زیادی را به خود جلب کرده است. در مطالعه حاضر، اثر ضدسرطانی نانوذرات نقره بیوسنتز شده با عصاره آبی و متانولی (80 درصد) جلبک سبز Spirogyra sp. بر روی سلول‌های سرطان پستان انسان رده MDA-MB-231 بررسی شد. کروماتوگرام طیف‌سنج GC-MS عصاره این جلبک نشان داد که ترکیبات Nonadecane (86/14 درصد)، Heneicosane (24/10 درصد) و Tridecane, 6-phenyl (44/5 درصد) بیشترین درصد را داشتند. نانوذرات نقره با افزودن عصاره آبی و متانولی جلبک به محلول نیترات نقره سنتز شد و ویژگی‌های فیزیکی و شیمیایی آن توسط روش­های اسپکتروفتومتری UV/Vis، FTIR، DLS، پتانسیل زتا و میکروسکوپ الکترونی FESEM تعیین شد. همچنین، سمیت سلولی نانوذره نقره بیوسنتز شده در غلظت­های مختلف با روش MTT بر روی سلول‌های سرطانی بررسی شد. نتایج نشان داد که نانوذرات نقره بیوسنتز شده دارای بیشترین جذب در طول موج 434 نانومتر بوده و میانگین اندازه آنها 30 تا 79 نانومتر با ریخت‌شناسی کروی شکل بودند. سمیت سلولی نانوذرات نقره بیوسنتزی علیه سلول­های سرطانی با افزایش غلظت بیشتر شد. در نتیجه نانوذرات نقره بیوسنتز شده به روش زیستی دارای فعالیت مهاری بر سرطان بودند و می­توانند در جهت درمان سرطان پستان مورد مطالعه بیشتری قرار گیرند.

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