اثرات پلی‌ساکارید فوکوئیدان استخراج شده از جلبک دریایی Cystoseira trinodis (C. Agardh, 1820) بر پاسخ‌های ایمنی میگوی وانامی Litopenaeus vannamei (Boone, 1931)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری زیست‌شناسی دریا، گروه زیست‌شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

2 دانشیار گروه زیست‌شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

3 استادیار گروه بیوتکنولوژی، پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

4 استادیار پژوهشکده تحقیقات میگوی کشور، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بوشهر، ایران.

5 دانشیار گروه فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی مازندران، ساری، ایران

چکیده

مطالعه حاضر، به منظور استفاده از جلبک دریایی خلیج فارس (جلبک قهوه‌ای Cystoseira trinodis) و همچنین استخراج پلی‌ساکارید فوکوئیدان به عنوان یک مکمل محرک ایمنی بر بهبود و ارتقای برخی شاخص‌های ایمنی میگوی وانامی انجام گرفت. از این رو، تعداد 500 قطعه میگو، با میانگین وزنی 8 گرم، به صورت طرح پایه کاملا تصادفی در چهار تیمار با سه تکرار (12 وان) تقسیم و به مدت 30 روز با جیره‌های غذایی حاوی صفر، 1/0، 2/0 و 4/0 درصد فوکوئیدان تغذیه شدند. نتایج نشان داد که تیمارهای فوکوئیدان تاثیر معنی‌داری بر شاخص‌های مورد سنجش داشتند (05/0P<). شاخص‌های ایمنی از جمله تعداد سلول‌های هیالین، گرانولار و هموسیت کل، آنیون سوپراکسید، فعالیت آنزیم‌های فنول اکسیداز، سوپراکسید دیسموتاز، کاتالاز و گلوتاتیون پراکسیداز در گروه‌های تغذیه شده با جیره غذایی حاوی سطوح مختلف فوکوئیدان بویژه 4/0 درصد در مقایسه با شاهد افزایش معنی‌داری داشت (05/0P<)، در حالی که مقدار مالون دی‌آلدهید کاهش معنی‌داری را نسبت به شاهد نشان داد (05/0P<). به طور کلی با توجه به نتایج مطالعه حاضر می‌توان اظهار داشت که فوکوئیدان استخراج شده از جلبک قهوه‌ای Cystoseira trinodis در سطوح بالاتر بویژه 4/0 درصد عملکرد بهتری در بهبود شاخص‌های ایمنی داشته است. از این رو، این ترکیب زیست فعال می‌تواند به عنوان محرک ایمنی به جیره غذایی میگو وانامی توصیه شود.

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